Clone:
Bio3-18E7
Type of antibody:
Secondary antibodies
Isotype:
mouse IgG1
Applications:
FC, IHC, ICC

Extended validation for Biotin Antibody

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
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Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
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Comparison of staining pattern on non-fixed and fixed cells using Biotin (Bio3-18E7). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
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Comparison of staining pattern on non-fixed and fixed cells using Biotin (Bio3-18E7). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using Biotin (Bio3-18E7). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for Biotin Antibody

Overview

Anti-Biotin fluorochromes are ideal for fluorescent staining of cells separated with Anti-Biotin MicroBeads, Streptavidin MicroBeads, or with the Anti-Biotin MultiSort Kit. They can also be used to stain cells labeled with cocktails of biotinylated antibodies, for example for quality control when using MACS
®
Cell Isolation Kits for the isolation of untouched cells. The Anti-Biotin antibody does not bind to free biotin which is often present in cell culture media.

Detailed product information

Technical specifications

CloneBio3-18E7
Clonalitymonoclonal
Isotypemouse IgG1
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodySecondary antibodies
Speciesany
AntigenBiotin
RRIDAB_2733612, AB_2726353, AB_2726076, AB_2726350, AB_2726073, AB_2726348, AB_2726071, AB_2733928, AB_2733929, AB_2726354, AB_2726077, AB_2733912, AB_2733913, AB_2732952, AB_2732953, AB_2726351, AB_2726074, AB_2726349, AB_2726072, AB_2726352, AB_2726075, AB_2726778, AB_2726694, AB_2733998, AB_2733999, AB_871546, AB_2733611

Resources for Biotin Antibody

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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