Streptavidin MicroBeads are used for the indirect magnetic labeling and separation of cells, subcellular material, or bacteria labeled with a biotinylated primary antibody or ligand.

Data and images for Streptavidin MicroBeads

Figures

Figure 1

Potato protoplasts isolated by MACS
®
Technology using biotinylated lectins and Streptavidin MicroBeads. (Courtesy of Dr. Dörr, Cologne, Germany.)
Positively selected protoplasts
Negative fraction
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Figure 1

Potato protoplasts isolated by MACS
®
Technology using biotinylated lectins and Streptavidin MicroBeads. (Courtesy of Dr. Dörr, Cologne, Germany.)
View details

Figure 1

Potato protoplasts isolated by MACS
®
Technology using biotinylated lectins and Streptavidin MicroBeads. (Courtesy of Dr. Dörr, Cologne, Germany.)

Figure 2

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Follicular dendritic cells isolated by MACS
®
Technology from human tonsils using Streptavidin MicroBeads. (Courtesy of Dr. J. Schmitz, Hamburg, Germany.)

Figure 2

Follicular dendritic cells isolated by MACS
®
Technology from human tonsils using Streptavidin MicroBeads. (Courtesy of Dr. J. Schmitz, Hamburg, Germany.)

Specifications for Streptavidin MicroBeads

Overview

Streptavidin MicroBeads are used for the indirect magnetic labeling and separation of cells, subcellular material, or bacteria labeled with a biotinylated primary antibody or ligand.

Detailed product information

Applications

Streptavidin MicroBeads have been used for the isolation of human allergen-specific B cells
1
or follicular dendritic cells
2
, murine liver sinusoidal endothelial cells
3
, sheep B cell subpopulations
4
, and rat retinal ganglion cells
5
. They have also been used in many other separations such as the isolation of plant protoplasts.
6

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Reviews for Streptavidin MicroBeads

Beads For Isolation Of Cells Labeled With Biotynilated Antibodies

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Streptavidin MicroBeads (130-048-102)

Our laboratory investigates different ways to improve immune responses against HIV. We investigate the effect of different cell population on the inhibition of HIV infection. We use this kit to purify (or deplete) different cell populations based on their surface marker. We like this kit for some less common markers because we do not need to buy expensive beads specific for each marker but can use the same beads for many different markers (mouse or human).

Streptavidin Beads

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Streptavidin MicroBeads (130-048-102)

Our lab performs many types of cell purifications from human and mouse cells for in vitro stimulation assays. Some purifications, such as depletion of all lineage committed cells, require an initial incubation with biotin-conjugated antibodies against many different cell markers. We use these beads as a second step for incubation and then separation of unlabelled cells.

References for Streptavidin MicroBeads

Publications

  1. Zürcher, A. W. et al. (1995) IgE-producing hybridomas established after B-cell culture in the CD40 system. Immunol. Lett. 46: 49-57
  2. Schmitz, J. et al. (1993) Optimizing follicular dendritic cell isolation by discontinuous gradient centrifugation and use of the magnetic cell sorter (MACS). J. Immunol. Methods 159: 189-196
  3. Do, H. et al. (1999) Expression of factor VIII by murine liver sinusoidal endothelial cells. J. Biol. Chem. 274: 19587-19592
  4. Gupta et al. (1998) Two B cell subpopulations have distinct recirculation characteristics. Eur. J. Immunol. 28: 1597-1603
  5. Shoge, K. et al. (1999) Rat retinal ganglion cells culture enriched with the magnetic cell sorter. Neurosci. Lett. 259: 111-114
  6. Dörr et al. (1994) Biotechnology 12: 511-515

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