Clone:
MB4-6D6
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
LCA, T200

Extended validation for CD45 Antibody, anti-non-human primate

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with MB4-6D6
5B1-
REA747-
2D1-
HI30-
REAL258-
REA1023++
Cells were incubated with an excess of purified unconjugated CD45 (MB4-6D6) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD45 Antibody, anti-non-human primate

Overview

The monoclonal CD45 antibody recognizes non-human primate leukocytes. The antibody reacts similar to human CD45 antibodies binding to the human leukocyte common antigen (LCA). CD45 is expressed on non-human primate lymphocytes, monocytes, and granulocytes. It is not expressed on erythroid cells.
The CD45 antibody recognizes the CD45 antigen of rhesus monkey (
Macaca mulatta
), cynomolgus monkey (
Macaca fascicularis
), and pigtail monkey (
Macaca nemestrina
) cells. Cross-reactivity with other non-human primates has not been tested. The antibody also recognizes the human CD45 antigen.

Alternative names

LCA, T200

Detailed product information

Technical specifications

CloneMB4-6D6
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Speciesnon-human primate, human
Cross-reactivityhuman
AntigenCD45
Alternative names of antigenLCA, T200
Molecular mass of antigen [kDa]41(
based on
Macaca mulatta
CD45
)
Distribution of antigenB cells, dendritic cells, granulocytes, Langerhans cells, macrophages, mast cells, monocytes, T cells, leukocytes, lymphocytes, platelets, basophils, thymocytes, hematopoietic stem and progenitor cells, plasmacytoid dendritic cells, plasma cells
RRIDAB_2751825, AB_2819549, AB_2819514, AB_2819557, AB_2819522, AB_244323, AB_2751856

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