The Anti-CLA MicroBead Kit was developed for the positive selection or depletion of human CLA
+
cells from PBMCs, lymphoid and non-lymphoid tissue for phenotypical and functional characterization.

Data and images for Anti-CLA MicroBead Kit, human

Figures

Figure 1

Isolation of CLA
+
cells from PBMCs using the Anti-CLA MicroBead Kit, a MiniMACS™ Separator, and an MS Column.
PBMCs before separation
CLA
-
cells
View details

Figure 1

Isolation of CLA
+
cells from PBMCs using the Anti-CLA MicroBead Kit, a MiniMACS™ Separator, and an MS Column.
View details

Figure 1

Isolation of CLA
+
cells from PBMCs using the Anti-CLA MicroBead Kit, a MiniMACS™ Separator, and an MS Column.
Isolated CLA
+
cells
View details

Figure 1

Isolation of CLA
+
cells from PBMCs using the Anti-CLA MicroBead Kit, a MiniMACS™ Separator, and an MS Column.

Specifications for Anti-CLA MicroBead Kit, human

Overview

The Anti-CLA MicroBead Kit was developed for the positive selection or depletion of human CLA
+
cells from PBMCs, lymphoid and non-lymphoid tissue for phenotypical and functional characterization.

Detailed product information

Background information

The cutaneous lymphocyte-associated antigen (CLA) is an inducible carbohydrate modification of P-selectin glycoprotein ligand 1 (PSGL-1) and functions as ligand for E-selectin. CLA is a skin-homing receptor and is found on a minor subset of human T cells that infiltrate the skin. This post-translational modification of PSGL-1 is discussed to serve as mechanism to regulate homing of CD4
+
and CD8
+
memory/effector T cells from peripheral blood to the skin; an immune response that plays an essential role during many inflammatory and certain malignant skin diseases.
In peripheral blood, CLA is expressed on skin-homing memory/effector T cells, on few B cells, NK cells, and almost all monocytes and dendritic cells, including Langerhans cells in the skin.
The Anti-CLA MicroBead Kit can be used in combination with MACS
®
Cell Isolation Kits or MACS MultiSort Kits, for example, with the CD8
+
T Cell Isolation Kit for the isolation of CD8
+
CLA
+
memory/effector T cells.

Detailed separation procedure

The isolation is performed by a two-step procedure. The CLA
+
cells are fluorescently and indirectly magnetically labeled with Anti-CLA-PE and Anti-PE MicroBeads.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

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