The Pan T Cell Isolation Kit has been developed for the faster isolation of untouched T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit gives you even higher purity, greater recovery, and faster cell isolation. You can isolate untouched T cells in as little as 18 minutes!

Data and images for Pan T Cell Isolation Kit, human

Figures

Figure 1

Separation of untouched T cells from PBMCs by depletion of non-T cells using the Pan T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were labeled with CD2-PE and CD3-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer.
PBMCs before separation
Untouched T cells
View details

Figure 1

Separation of untouched T cells from PBMCs by depletion of non-T cells using the Pan T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were labeled with CD2-PE and CD3-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer.
View details

Figure 1

Separation of untouched T cells from PBMCs by depletion of non-T cells using the Pan T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were labeled with CD2-PE and CD3-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer.

Figure 2

View details
Isolation of untouched T cells.

Figure 2

Isolation of untouched T cells.

Specifications for Pan T Cell Isolation Kit, human

Overview

The Pan T Cell Isolation Kit has been developed for the faster isolation of untouched T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit gives you even higher purity, greater recovery, and faster cell isolation. You can isolate untouched T cells in as little as 18 minutes!

Detailed product information

Detailed separation procedure

Non-target cells, i.e., monocytes, neutrophils, eosinophils, B cells, stem cells, dendritic cells, NK cells, granulocytes, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, and CD235a (Glycophorin A). Subsequently, non-target cells are magnetically labelled with the Pan T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.

Downstream applications

T cells isolated with the Pan T Cell Isolation Kit can be used when direct labeling of T cell surface molecules could interfere with downstream applications, e.g., in studies on T cell activation.
1, 2
T cells isolated with the Pan T Cell Isolation Kit have also been used to generate antigen-specific T cell lines.
3, 4
Untouched T cells may also be used for further isolation of specific T cell subsets, such as naive
5
or memory T cells and activated T cells.

Columns

LS or autoMACS
®
Columns.

References for Pan T Cell Isolation Kit, human

Publications

  1. Wagner, M. et al. (2004) IL-12p70-dependent Th1 induction by human B cells requires combined activation with CD40 ligand and CpG DNA. J. Immunol. 172: 954-963
  2. Finney, H. M. et al. (2004) Activation of resting human primary T cells with chimeric receptors: costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCR zeta chain. J. Immunol. 172: 104-113
  3. Faure, S. et al. (2004) ERM proteins regulate cytoskeleton relaxation promoting T cell-APC conjugation. Nat. Immunol. 5: 272-279
  4. Bukczynski, J. et al. (2004) Costimulatory ligand 4-1BBL (CD137L) as an efficient adjuvant for human antiviral cytotoxic T cell responses. Proc. Natl. Acad. Sci. U.S.A. 101: 1291-1296
  5. Hafsi, N. et al. (2004)
    Human dendritic cells respond to
    Helicobacter pylori
    , promoting NK cell and Tʜ1-effector responses
    in vitro
    .
    J. Immunol. 173: 1249-1257

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