CD45RO MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs.

Data and images for CD45RO MicroBeads, human

Figures

Figure 1

CD4
+
cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4
+
CD45RO
+
effector and memory T helper cells were isolated by positive selection.
Pre-enriched CD4
+
cells
CD4
+
CD45RO
+
cells
View details

Figure 1

CD4
+
cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4
+
CD45RO
+
effector and memory T helper cells were isolated by positive selection.
View details

Figure 1

CD4
+
cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4
+
CD45RO
+
effector and memory T helper cells were isolated by positive selection.

Specifications for CD45RO MicroBeads, human

Overview

CD45RO MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs.

Detailed product information

Background information

CD45RO is expressed on CD4
+
and CD8
+
T memory cells as well as on CD4
+
effector T cells. CD45RO is also expressed on monocytes, macrophages, and granulocytes. The CD45RO antibody recognizes a 180 kDa isoform of the leukocyte common antigen (LCA).
CD45RO MicroBeads can be used for positive selection or depletion of CD45RO
+
cells. For isolation of CD45RO
+
T cell subsets, the CD45RO MicroBeads can be combined with CD4 and CD8 MACS® T Cell Isolation Kits
1,2,7,8
or MultiSort Kits
3,4
. The CD45RO MicroBeads can also be used to isolate CD45RA
+
cells from pre-sorted CD4
+
or CD8
+
T cells by depletion of CD45RO
+
cells.
5,7,8

Downstream applications

Cells isolated with CD45RO MicroBeads can be used for various studies, such as cytokine expression
6
or receptor signaling.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for CD45RO MicroBeads, human

Publications

  1. Krug et al. (2001) Toll-like receptor expression reveals CpG DNA as a unique microbial stimulus for plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12. Eur. J. Immunol. 31: 3026-3037
  2. Coccia, E. M. et al. (1999) Interleukin-12 induces expression of interferon regulatory factor-1 via signal transducer and activator of transcription-4 in human T helper type 1 cells. J. Biol. Chem. 274: 6698-6703
  3. Wolthers, K. C. et al. (1999) Normal T-cell telomerase activity and upregulation in human immunodeficiency virus-1 infection. Blood 93: 1011-1019
  4. Jonuleit et al. (2001)
    Identification and functional characterization of human CD4
    +
    CD25
    +
    T cells with regulatory properties isolated from peripheral blood.
    J. Exp. Med. 193: 1285-1294
  5. Kimmig, S. et al. (2002) Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med. 195: 789-794
  6. Malmberg, K. J. et al. (2001)
    Inhibition of activated/memory (CD45RO
    +
    ) T cells by oxidative stress associated with block of NF-kappaB activation.
    J. Immunol. 167: 2595-2601
  7. Wagner, M. et al. (2004) IL-12p70-dependent Th1 induction by human B cells requires combined activation with CD40 ligand and CpG DNA. J. Immunol. 172: 954-963
  8. Verhasselt et al. (2004) Induction of FOXP3-expressing regulatory CD4pos T cells by human mature autologous dendritic cells. Eur. J. Immunol. 34: 762-772

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