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CD4 + cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4 +CD45RO + effector and memory T helper cells were isolated by positive selection. |
Pre-enriched CD4 + cells | CD4 +CD45RO + cells |
CD45RO MicroBeads, humanFigure 1CD4 + cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4 +CD45RO + effector and memory T helper cells were isolated by positive selection. | CD45RO MicroBeads, humanFigure 1CD4 + cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4 +CD45RO + effector and memory T helper cells were isolated by positive selection. |
CD4 + cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4 +CD45RO + effector and memory T helper cells were isolated by positive selection. |
Pre-enriched CD4 + cells | CD4 +CD45RO + cells |
CD45RO MicroBeads, humanFigure 1CD4 + cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4 +CD45RO + effector and memory T helper cells were isolated by positive selection. | CD45RO MicroBeads, humanFigure 1CD4 + cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4 +CD45RO + effector and memory T helper cells were isolated by positive selection. |
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