CD8
⁺
T Cell Isolation Kit
, human

Name: CD8+ T Cell Isolation Kit, human
Availability: InStock

CD8
⁺
T Cell Isolation Kit
, human

The CD8

⁺

T Cell Isolation Kit has been developed for fast isolation of untouched cytotoxic CD8

⁺

T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit offers even better performance and a significantly shorter protocol and replaces the previous kit (#130-094-156).

Product data and images for CD8
⁺
T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD8

⁺

T cells were isolated from human PBMCs using the CD8

⁺

T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8

⁺

T cells were CD3

⁺

and CD56

^–

. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer.

Unseparated fraction

Figure 1

Untouched CD8

⁺

T cells were isolated from human PBMCs using the CD8

⁺

T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8

⁺

T cells were CD3

⁺

and CD56

^–

. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer.

Figure 1

Untouched CD8

⁺

T cells were isolated from human PBMCs using the CD8

⁺

T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8

⁺

T cells were CD3

⁺

and CD56

^–

. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer.

Isolated CD8⁺ T cells

Figure 1

Untouched CD8

⁺

T cells were isolated from human PBMCs using the CD8

⁺

T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8

⁺

T cells were CD3

⁺

and CD56

^–

. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer.

Figure 1

Untouched CD8

⁺

T cells were isolated from human PBMCs using the CD8

⁺

T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8

⁺

T cells were CD3

⁺

and CD56

^–

. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer.

Figure 2

Figure 2

Specifications for CD8
⁺
T Cell Isolation Kit
, human

Overview

The CD8

⁺

T Cell Isolation Kit has been developed for fast isolation of untouched cytotoxic CD8

⁺

T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit offers even better performance and a significantly shorter protocol and replaces the previous kit (#130-094-156).

Detailed product information

Detailed separation procedure

Non-CD8

⁺

cells, i.e., CD4

⁺

T cells, monocytes, neutrophils, eosinophils, B cells, stem cells, dendritic cells, NK cells, granulocytes, γ/δ T cells, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD4, CD15, CD16, CD19, CD34, CD36, CD56, CD123, TCRγ/δ, and CD235a (Glycophorin A). Subsequently, non-target cells are magnetically labelled with the CD8

⁺

T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.

Downstream applications

The CD8

⁺

T Cell Isolation Kit is the tool of choice when direct labeling of CD8

⁺

T cell surface molecules could interfere with downstream applications, e.g., in studies on cytotoxic T cell activation

¹

. Untouched CD8

⁺

T cells may also be used for the isolation of specific cytotoxic T cell subsets, such as naive or memory CD8

⁺

T cells and activated CD8

⁺

T cells.

Columns

LS or autoMACS

^®

Columns.

Resources for CD8
⁺
T Cell Isolation Kit
, human

Documents and Protocols

Brochures/posters

MACS® Cell Pre-Enrichment for flow sorting and analysis

Brochures/posters

T cell research - The complete product portfolio

Scientific posters

Fully automated clinical-grade isolation of CD141 (BDCA-3)⁺ cross-presenting dendritic cells

App notes/customer reports

Antigen-specific restimulation of CD8+ T cells by cross-presenting dendritic cells

Protocols

Isolation of CD8⁺CD62L⁺ T cells from human PBMCs

References for CD8
⁺
T Cell Isolation Kit
, human

Publications

Finney, H. M. et al. (2004) Activation of resting human primary T cells with chimeric receptors: costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCR zeta chain. J Immunol 172: 104-113

Data and images