Clone:
HECA-452
Type of antibody:
Primary antibodies
Isotype:
rat IgMκ
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
SELPLG, CD162, PSGL-1, PSGL1

Extended validation for CLA Antibody, anti-human

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CLA (HECA-452). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CLA (HECA-452). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CLA (HECA-452). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CLA Antibody, anti-human

Overview

The Anti-CLA antibody recognizes the cutaneous lymphocyte-associated antigen (CLA), a specialized glycoform of P-selectin glycoprotein ligand-1 (PSGL-1). It serves as a ligand for selectins, including CD62E (ELAM-1) and CD62L (LECAM-1). CLA is a unique skin-homing receptor and is predominantly found on a minor subset of human T cells that infiltrate the skin. This post-translational modification of PSGL-1 is thought to serve as a mechanism to regulate tissue-specific homing of CD4
+
and CD8
+
memory/effector T cells from peripheral blood to the skin, which plays an essential role during many inflammatory and certain malignant skin diseases.
In peripheral blood, CLA is not only found on skin-homing 
memory/effector T cells, but is also found to be expressed on memory/effector B cells, NK cells, blood dendritic cells, and on monocytes. CLA is furthermore found on Langerhans cells in the skin.

Alternative names

SELPLG, CD162, PSGL-1, PSGL1

Detailed product information

Technical specifications

CloneHECA-452
Clonalitymonoclonal
Isotyperat IgMκ
Isotype controlIsotype Control Antibody, rat IgM
Hostrat
Type of antibodyPrimary antibodies
Specieshuman
AntigenCLA
Alternative names of antigenSELPLG, CD162, PSGL-1, PSGL1
Molecular mass of antigen [kDa]39
Entrez Gene ID6404
RRIDAB_2857620, AB_2659951, AB_615108, AB_2659954, AB_2659955, AB_2659956, AB_2659957, AB_615106, AB_615105, AB_2857648

References for CLA Antibody, anti-human

Publications

  1. Santamaria Babi, L. F. et al. (1995) Migration of skin-homing T cells across cytokine-activated human endothelial cell layers involves interaction of the cutaneous lymphocyte-associated antigen (CLA), the very late antigen-4 (VLA-4), and the lymphocyte function-associated antigen-1 (LFA-1). J. Immunol. 154: 1543-1550
  2. Takahashi, R. et al. (2003)
    In vitro
    differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
    J. Immunol. 171: 5769-5777
  3. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
  4. Fuhlbrigge, R. C. et al. (1997) Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells. Nature 389: 978-981
  5. Strunk, D. et al. (1997) A skin homing molecule defines the Langerhans cell progenitor in human peripheral blood. J. Exp. Med. 185: 1131-1136
  6. Tsuchiyama, J. et al. (2002) Induction and characterization of cutaneous lymphocyte antigen on natural killer cells. Br J Haematol 118: 654-662
  7. Yoshino, T. et al. (1999) Cutaneous lymphocyte antigen is expressed on memory/effector B cells in the peripheral blood and monocytoid B cells in the lymphoid tissues. Cell. Immunol. 197: 39-45
  8. Bos, J. D. et al. (1993) Skin-homing T lymphocytes: detection of cutaneous lymphocyte-associated antigen (CLA) by HECA.452 in normal human skin. Arch. Dermatol. Res. 285(179): 179-183

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