StainExpress™ CAR T Cell Transduction Cocktail provides a preformulated antibody backbone panel to simplify flow cytometric evaluation of transduction efficiency in cell manufacturing of chimeric antigen receptor (CAR)
+
T cells. This unified backbone panel is designed for immunofluorescent staining of cell manufacturing samples, peripheral mononuclear cells (PBMCs), or other lysed whole blood samples, to identify the CAR
+
cells among CD3
+
T cells for a CAR of choice.

Specifications for StainExpress™ CAR T Transduction Cocktail, human

Overview

StainExpress™ CAR T Cell Transduction Cocktail provides a preformulated antibody backbone panel to simplify flow cytometric evaluation of transduction efficiency in cell manufacturing of chimeric antigen receptor (CAR)
+
T cells. This unified backbone panel is designed for immunofluorescent staining of cell manufacturing samples, peripheral mononuclear cells (PBMCs), or other lysed whole blood samples, to identify the CAR
+
cells among CD3
+
T cells for a CAR of choice.

Detailed product information

Background information

The StainExpress CAR T Cell Transduction Cocktail is a unified backbone panel to identify CD3
+
T cells, discriminate CAR
-
and CAR
+
cells, and divide them into CD4
+
and CD8
+
T cells. It is therefore designed to include a CAR detection reagent of choice for detection in the free PE channel. Miltenyi Biotec's CAR detection reagents, for example, have been developed for PE channel-detection of transduced T cells that are engineered to express specific CARs on the cell surface. The cocktail offers consistent formulation in carefully dried down cocktails containing our REAfinity™ Recombinant Antibodies.
REAfinity Antibodies are generated in a biologically and chemically defined in vitro expression system to guarantee maximum purity and lot-to-lot consistency. The genetic sequence is engineered to lack binding to Fcγ receptors (FcγRs). Binding to FcγRs is one of the main causes of background signal in flow cytometry analysis.
Flow cytometric analysis of the Immune Cell Composition Cocktail can be conducted on several flow cytometers equipped with a red (638 nm), a blue (488 nm), and a violet (405 nm) laser, for example, the MACSQuant® Analyzer 10 or MACSQuant Analyzer 16.

Applications

  • Evaluation of the transduction efficiency in CAR T cell manufacturing
  • Identification of CAR+ T cells by flow cytometry

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