PepTivator
®
SARS-CoV-2 Select – premium grade is a peptide pool of 88 lyophilized oligopeptides, consisting of MHC class I and class II-restricted peptides derived from the whole proteome of SARS-CoV-2.
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Specifications for
PepTivator
®
SARS-CoV-2 Select

Overview

PepTivator
®
SARS-CoV-2 Select – premium grade is a peptide pool of 88 lyophilized oligopeptides, consisting of MHC class I and class II-restricted peptides derived from the whole proteome of SARS-CoV-2.
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Detailed product information

Background information

SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), also known as novel coronavirus 2019-nCoV, causes fever, severe respiratory illness and can lead to life threatening pneumonia. The first cases of this disease, termed COVID-19 for coronavirus disease 2019, have been detected in December 2019 in Wuhan, China.
The PepTivator
®
SARS-CoV-2 Select pool contains 88 peptides of 9 – 22 aa in length. 63 of these peptides are MHC class I-restricted and 25 peptides are MHC class II-restricted. The peptides originate from structural proteins (S, M, N, E) as well as non-structural proteins.
The peptides are restricted to the following HLA-molecules: HLA-A*01, A*02, A*03, A*11, A*24, A*29, A*30, A*31, A*68, B*07, B*08, B*15, B*27, B*35, B*40, B*44, B*54, B*58, C*07, DRB1*01, DRB1*03, DRB1*04, DRB1*07, DRB1*11, DRB1*15.

Applications

The in vitro stimulation of SARS-CoV-2–specific CD4
+
and CD8
+
T cells with PepTivator
®
SARS-CoV-2 Select causes secretion of effector cytokines and upregulation of activation markers, which then allow the detection and isolation of SARS-CoV-2–specific T cells:
Detection and analysis of antigen-specific CD4
+
and CD8
+
effector/memory T cells in PBMCs by MACS
®
Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
Isolation of viable antigen-specific CD4
+
T cells with the CD154 MicroBead Kit, or of viable CD4
+
and CD8
+
T cells using MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
Generation antigen-specific CD4
+
and CD8
+
effector/ memory T cells from naive T cell populations.
Pulsing of antigen-presenting cells e.g. for research on dendritic cell vaccination.

Resources for
PepTivator
®
SARS-CoV-2 Select

References for
PepTivator
®
SARS-CoV-2 Select

Publications

  1. Anft, M. et al. (2020) COVID-19 progression is potentially driven by T cell immunopathogenesis medRxiv - the preprint server for health sciences (doi.org/10.1101/2020.04.28.20083089 )
  2. Thieme, C. J. et al. (2020) Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients. Cell Rep Med. 1(6): 100092
  3. Tamminen, K. et al. (2021) Fusion Protein of Rotavirus VP6 and SARS-CoV-2 Receptor Binding Domain Induces T Cell Responses Vaccines (Basel) 9(7): 733

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PepTivator
®
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