Clone:
REA140
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC
Alternative names:
ALL3, BSAP, EBB-1, KLP

Extended validation for PAX-5 (BSAP) Antibody, anti-human/mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA140
1H9++
Cells were incubated with an excess of purified unconjugated PAX-5 (BSAP) (REA140) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for PAX-5 (BSAP) Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA140 recognizes PAX-5, which belongs to the paired box (PAX) gene family of transcription factors. PAX-5 is the only mammalian PAX transcription factor, which is expressed in hematopoietic cells. Within the hematopoietic cells, expression of PAX-5 is limited to cells of the B-lymphocyte lineage and it exerts transcriptional control of B cell commitment, development, and tumorigenesis. PAX-5 contains DNA binding conserved ‘paired’ domain, which consists of an N- and a C-terminal subdomain. Each subregion contains a homeodomain-like helix-turn-helix motif, which binds to a half-site of the PAX-5 recognition sequence. Depending upon the interaction partners, PAX-5 serves either as an activator or repressor of transcription. For instance, Groucho protein family members, by binding to a conserved octapeptide motif of PAX-5, enable PAX-5 to serve as a repressor.
Additional information: Clone REA140 displays negligible binding to Fc receptors.

Alternative names

ALL3, BSAP, EBB-1, KLP

Detailed product information

Technical specifications

CloneREA140
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenPAX-5 (BSAP)
Alternative names of antigenALL3, BSAP, EBB-1, KLP
Distribution of antigenB cells
RRIDAB_2653160, AB_2653161, AB_2653162, AB_2653163, AB_2653164, AB_2653165

Resources for PAX-5 (BSAP) Antibody, anti-human/mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for PAX-5 (BSAP) Antibody, anti-human/mouse, REAfinity™

Publications

  1. Pridans, C. et al. (2008) Identification of Pax5 target genes in early B cell differentiation. J. Immunol. 180: 1719-1728
  2. Fazio, G. et al. (2008) PAX5/TEL acts as a transcriptional repressor causing down-modulation of CD19, enhances migration to CXCL12, and confers survival advantage in pre-BI cells. Cancer Res. 68: 181-189
  3. He, T., et al. (2011) Histone acetyltransferase p300 acetylates Pax5 and strongly enhances Pax5-mediated transcriptional activity. J. Biol. Chem. 286: 14137-14145

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