Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines.
ODN 2006 is a B-class ODN for activation of B cells , induction of Il-6 secretion and activation of NF-kB-signalling pathways.

Specifications for ODN 2006

Overview

Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines.
ODN 2006 is a B-class ODN for activation of B cells , induction of Il-6 secretion and activation of NF-kB-signalling pathways.

Detailed product information

Background information

TLR9 is a prominent member of the toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns. TLR9 recognizes specifically unmethylated CpG motifs in bacterial DNA leading to activation of immune cells
1
,
2
. These effects can be mimicked by short synthetical oligodeoxyribonucleotides (ODNs) containing unmethylated CpG motifs
3
.
Several classes of CpG ODN have been identified and can be distinguished by their effects on certain cell types. A-class ODNs containing 5’ and 3’ G-rich stretches induce high levels of type I IFN but show low activation of B cell proliferation. B-class ODNs activate B cells and TLR9-dependent NFkB signalling in recombinant cell lines but show low induction of IFN-α. C-class ODN induce high amounts of IFN-α and activate B cells. The recently discovered new P-Class ODNs show similar but superior properties to C-class ODNs.

Applications

B-class ODN for activation of B cells , induction of Il-6 secretion from human PBMCs, and activation of NF-kB-signalling pathways.

Resources for ODN 2006

Documents and Protocols

References for ODN 2006

Publications

  1. Hemmi, H. et al. (2000) A Toll-like receptor recognizes bacterial DNA. Nature 408: 740-745
  2. Krieg, A. M. et al. (1995) CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 374: 546-549
  3. Bauer, S. et al. (2001) Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc. Natl. Acad. Sci. U.S.A. 98: 9237-9242

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