Clone:
REA1014
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
IGHA1, IgA1, IgA2

Extended validation for IgA Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1014
B3506B4-
IS11-8E10++
Cells were incubated with an excess of purified unconjugated IgA (REA1014) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-IgA. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgA antibodies and with a suitable counterstaining. As a control, Anti-IgA antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-IgA. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgA antibodies and with a suitable counterstaining. As a control, Anti-IgA antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-IgA. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgA antibodies and with a suitable counterstaining. As a control, Anti-IgA antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-IgA. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgA antibodies and with a suitable counterstaining. As a control, Anti-IgA antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-IgA. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgA antibodies and with a suitable counterstaining. As a control, Anti-IgA antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using IgA (REA1014). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using IgA (REA1014). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using IgA (REA1014). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for IgA Antibody, anti-human, REAfinity™

Overview

Clone REA1014 detects both subclasses of human IgA. IgA is present either as monomer or in a secreted form as a multimer of 2–4 molecules, connected by the J-chain and the so-called secretory component. Secreted IgA is transported across epithelial cells and secreted into the lumen of the respiratory and gastrointestinal tract.
Additional information: Clone REA1014 displays negligible binding to Fc receptors.

Alternative names

IGHA1, IgA1, IgA2

Detailed product information

Technical specifications

CloneREA1014
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenIgA
Alternative names of antigenIGHA1, IgA1, IgA2
Molecular mass of antigen [kDa]38
Distribution of antigenB cells
Entrez Gene ID3493
RRIDAB_2727737, AB_2727808, AB_2727738, AB_2727809, AB_2727739, AB_2727810, AB_2727740, AB_2727811, AB_2727741, AB_2727812, AB_2727742, AB_2727813, AB_2727743, AB_2727814, AB_2727744, AB_2727815, AB_2727745, AB_2801914, AB_2727807

Resources for IgA Antibody, anti-human, REAfinity™

Certificates

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