Clone:
REA385
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, ICC, MC
Alternative names:
CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28, RBS11

Extended validation for CX3CR1 Antibody, anti-human, REAfinity™

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CX3CR1 (REA385). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CX3CR1 (REA385). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CX3CR1 (REA385). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CX3CR1 Antibody, anti-human, REAfinity™

Overview

Clone REA385 recognizes the human CX3C chemokine receptor 1 antigen, a 40 kDa G-protein–coupled multi-pass membrane protein also known as fractalkine receptor. CX3CR1 is expressed on natural killer (NK) cells, T cell subsets, monocytes/macrophages, dendritic cells (DCs), and some malignant epithelial cells. Binding of CX3CR1 to the membrane-bound form of the chemokine CX3CL1 promotes cell-cell adhesion, whereas the soluble form induces cell migration of CX3CR1-bearing cells such as monocytes, NK cells, T cells, DCs, and macrophages including microglia. CX3CR1 plays also an important role in the formation of transepithelial dendrites by intestinal DCs. Failure in the CX3CL1/CX3CR1 interaction may contribute to the development of several inflammatory diseases including atherosclerosis, psoriasis, rheumatoid arthritis, and experimental autoimmune myositis.
Additional information: Clone REA385 displays negligible binding to Fc receptors.

Alternative names

CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28, RBS11

Detailed product information

Technical specifications

CloneREA385
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCX3CR1
Alternative names of antigenCCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28, RBS11
Molecular mass of antigen [kDa]40
Distribution of antigenNK cells, T cells, dendritic cells, macrophages, monocytes, epithelial cells
Entrez Gene ID1524
RRIDAB_2751738, AB_2801984, AB_2801963, AB_2801867, AB_2751798

Resources for CX3CR1 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CX3CR1 Antibody, anti-human, REAfinity™

Publications

  1. Niess, J. H. et al. (2005)
    CX
    3
    CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance.
    Science 307: 254-258
  2. Imai, T. et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 91: 521-530
  3. Raport, C. J. et al. (1995) The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 163(2): 295-299

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