Clone:
REAL163
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC
Alternative names:
L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL, Lnhr, LSEL, Lyam1, PLNHR

Extended validation for CD62L Antibody, anti-human,
REAlease
®

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REAL163
SK11++
145/15++
REA615++
Cells were incubated with an excess of purified unconjugated CD62L (REAL163) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD62L Antibody, anti-human,
REAlease
®

Overview

Clone REAL163 is an antibody fragment derived from the full CD62L antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL163 recognizes the human CD62L antigen, a 74 kDa single-pass type I membrane protein which is also known as L-selectin, LECAM-1, or LAM-1. CD62L is a member of the selectin family of cell surface molecules and binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. Most hematopoietic cells express CD62L, including peripheral blood B cells, T cells, monocytes, granulocytes, thymocytes, and some myeloid cells from bone marrow. Expression on resting naive, central memory, and some effector memory T cells regulates their migration via endothelial venules to peripheral lymph nodes and Peyer’s patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Disruption of CD62L expression has detrimental effects on T cell migration and immune responses. Antigenic activation in the lymph node leads first to rapid CD62L shedding by protease cleavage and then to transcriptional suppression.
Always use fresh material for immunofluorescent staining of CD62L
+
cells. For optimal results, the cells should not be older than 8–12 hours. Keep cells continuously cold. CD62L-expression may be rapidly lost due to shedding.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL, Lnhr, LSEL, Lyam1, PLNHR

Detailed product information

Technical specifications

CloneREAL163
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
,
rhesus monkey (
Macaca mulatta
)
, baboon
AntigenCD62L
Alternative names of antigenL-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL, Lnhr, LSEL, Lyam1, PLNHR
Distribution of antigenB cells, T cells, myeloid cells, granulocytes, monocytes
RRIDAB_2751070, AB_2751435, AB_2751417, AB_2751101, AB_2751082, AB_2751273, AB_2751267, AB_2784262, AB_2784263, AB_2784261, AB_2784264, AB_2751094

Resources for CD62L Antibody, anti-human,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD62L Antibody, anti-human,
REAlease
®

Publications

  1. Ivetic, A. (2013) Signals regulating L-selectin-dependent leucocyte adhesion and transmigration. Int. J. Biochem. Cell Biol. 45(3): 550-555
  2. Telen, M. J. (2014) Cellular adhesion and the endothelium: E-selectin, L-selectin, and pan-selectin inhibitors. Hematol. Oncol. Clin. North Am. 28(2): 341-354
  3. McEver, R. P. (2015) Selectins: initiators of leucocyte adhesion and signalling at the vascular wall. Cardiovasc. Res. 107(3): 331-339

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CD62L Antibody, anti-human,
REAlease
®

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