Clone:
REAL274
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
NCAM1, MSK39, NCAM

Extended validation for CD56 Antibody, anti-human,
REAlease
®

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD56 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD56-PE (REAL274, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD56 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD56-PE (REAL274, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD56 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD56-PE (REAL274, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD56 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD56-PE, clone (REAL274). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD56 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD56-PE, clone (REAL274). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD56 Antibody, anti-human,
REAlease
®

Overview

Clone REAL274 is an antibody fragment derived from the full CD56 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL274 recognizes the human CD56 antigen, a glycoprotein of the Ig-superfamily, also known as neural cell adhesion molecule (NCAM), which is expressed in blood on practically all resting and activated NK cells and on a minor subset of CD3
+
T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal-derived tumors, myelomas, and myeloid leukemias also express CD56.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

NCAM1, MSK39, NCAM

Detailed product information

Technical specifications

CloneREAL274
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD56
Alternative names of antigenNCAM1, MSK39, NCAM
Distribution of antigenNK cells, T cells, brain
RRIDAB_2751326, AB_2751437, AB_2751419, AB_2751331, AB_2751324, AB_2784257, AB_2784258, AB_2784256, AB_2751333

Resources for CD56 Antibody, anti-human,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD56 Antibody, anti-human,
REAlease
®

Publications

  1. Camp, B. V. et al. (1990) Plasma cells in multiple myeloma express a natural killer cell- associated antigen: CD56 (NKH-1; Leu-19). Blood 76: 377-382

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