Clone:
REA364
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IHC, IF
Alternative names:
Spn, B-cell differentiation antigen LP-3, GALGP, Ly-48, Sialophorin, CD43

Specifications for CD43 Glycoform Antibody, anti-mouse, REAfinity™

Overview

Clone REA364 recognizes the activation-associated glycoform (130-kD glycoform) of the murine CD43 antigen, a cell-surface glycoprotein which is also known as sialophorin or leukosialin. CD43 is a heavily O-glycosylated protein which is expressed in a variety of hematopoietic lineage cells, including T lymphocytes. It has been reported to have two glycoforms, one with a molecular mass of 115 kD that is expressed on all T cells and one with a mass of 130 kD that is expressed only on activated T cells. Biochemical analysis showed that CD43 on resting T cells carries tetrasaccharides (115-kD glycoform), whereas activated T cells carry branched hexasaccharide core two O-glycans (130-kD glycoform). Expression of the 130-kD glycoform of CD43 on all T cells in transgenic mice shows a reduction in primary T-cell immune responses that is probably due to the antiadhesive function of this glycoform.
Additional information: Clone REA364 displays negligible binding to Fc receptors.

Alternative names

Spn, B-cell differentiation antigen LP-3, GALGP, Ly-48, Sialophorin, CD43

Detailed product information

Technical specifications

CloneREA364
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD43 Glycoform
Alternative names of antigenSpn, B-cell differentiation antigen LP-3, GALGP, Ly-48, Sialophorin, CD43
Molecular mass of antigen [kDa]38
Distribution of antigenB cells, T cells, lymphocytes
Entrez Gene ID20737
RRIDAB_2658142, AB_2658143, AB_2658144, AB_2658145, AB_2658148, AB_2658149

Resources for CD43 Glycoform Antibody, anti-mouse, REAfinity™

Certificates

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References for CD43 Glycoform Antibody, anti-mouse, REAfinity™

Publications

  1. Carlow, D. A. et al. (1999) A novel CD8 T cell-restricted CD45RB epitope shared by CD43 is differentially affected by glycosylation J. Immunol. 163(3): 1441-1448
  2. van den Berg, T. K. et al. (2001) Cutting Edge: CD43 functions as a T cell counterreceptor for the macrophage adhesion receptor Sialoadhesin (Siglec-1). J. Immunol. 166: 3637- 3640
  3. Alcaide, P. et al. (2007)
    The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Tʜ1 cells
    in vitro
    and in delayed-type hypersensitivity reactions
    in vivo.
    J. Invest. Dermatol. 127(8): 1964-1972

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