Clone:
HEA-125
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
EGP40, Ep-CAM, KSA, MK-1, TROP1

Extended validation for CD326 (EpCAM) Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with HEA-125
9C4++
EBA-1++
CO17-1A++
1B7++
VU-1D9++
REA764++
REAL546++
Cells were incubated with an excess of purified unconjugated CD326 (EpCAM) (HEA-125) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD326 (EpCAM) Antibody, anti-human

Overview

Clone HEA-125 recognizes the CD326 antigen, also known as human epithelial antigen (HEA), epithelial cell adhesion molecule (EpCAM), or epithelial-specific antigen (ESA), and is involved in cell adhesion. The CD326 antigen is broadly expressed on the basolateral surface of carcinoma and epithelial cells in tissues or on circulating tumor cells and cancer stem cells, but is not found on melanoma, neuroblastoma, sarcoma, lymphoma, leukemia cells, or normal fibroblasts.

Alternative names

EGP40, Ep-CAM, KSA, MK-1, TROP1

Detailed product information

Technical specifications

CloneHEA-125
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD326 (EpCAM)
Alternative names of antigenEGP40, Ep-CAM, KSA, MK-1, TROP1
Molecular mass of antigen [kDa]33
Distribution of antigenstem cells, epithelial cells, cancer stem cells, ES and iPS cells, lung
Entrez Gene ID4072
RRIDAB_2726064, AB_2726342, AB_2726065, AB_2726338, AB_2726061, AB_2733940, AB_2733941, AB_2726776, AB_2726692, AB_2726343, AB_2726066, AB_2726339, AB_2726062, AB_2726344, AB_2726067, AB_2751175, AB_2726340, AB_2726063, AB_2751108, AB_2726341

Resources for CD326 (EpCAM) Antibody, anti-human

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD326 (EpCAM) Antibody, anti-human

Publications

  1. Moldenhauer, G. et al. (1987) Epithelium specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br. J. Cancer 56: 714-721
  2. Metsuyanim, S. et al. (2009) Expression of stem cell markers in the human fetal kidney. PLoS One 4(68): e6709
  3. Sheridan, C. et al. (2006)
    CD44
    +
    /CD24
    -
    breast cancer cells exhibit enhanced invasive properties: an early step necessary for metastasis.
    Breast Cancer Res. 8(5): R59

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