Clone:
REA109
Type of antibody:
Primary antibodies, Recombinant antibodies, Functional-grade antibodies
Isotype:
recombinant human IgG1
Applications:
FC, FA, MC
Alternative names:
TLR2, TIL4, Ly105

Extended validation for CD282 (TLR2) Antibody, anti-human/mouse, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD282 (TLR2). Human peripheral blood mononuclear cells (PBMCs) were stained with CD282 (TLR2) antibodies and with a suitable counterstaining. As a control, CD282 (TLR2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD282 (TLR2). Human peripheral blood mononuclear cells (PBMCs) were stained with CD282 (TLR2) antibodies and with a suitable counterstaining. As a control, CD282 (TLR2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD282 (TLR2). Human peripheral blood mononuclear cells (PBMCs) were stained with CD282 (TLR2) antibodies and with a suitable counterstaining. As a control, CD282 (TLR2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD282 (TLR2). Human peripheral blood mononuclear cells (PBMCs) were stained with CD282 (TLR2) antibodies and with a suitable counterstaining. As a control, CD282 (TLR2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD282 (TLR2) (REA109). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD282 (TLR2) (REA109). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD282 (TLR2) (REA109). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD282 (TLR2) Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA109 recognises human and mouse CD282, also known as toll-like receptor 2 (TLR2). TLRs are the host pattern recognition receptors involved in recognising pathogen associated and certain tissue damage indicating molecules. They are type 1 transmembrane proteins with extracellular leucine-rich repeats and intracellular toll/IL-1 receptor domains. TLR2 is a plasma membrane associated TLR and recognizes various structurally diverse molecules including peptidoglycans (PG), lipopeptides and lipoproteins moieties in Gram
+
and Gram
bacteria, lipoteichoic acid (LTA) in Gram
+
bacteria, lipoarabinomannan and lipomannan from Mycoplasma, and fungal polysaccharides such as zymogen. High-resolution crystal structures suggested that TLR2 by forming heterodimers with TLR6 and TLR1 can distinguish between di-acylated and tri-acylated lipopeptides. The expression of CD282 (TLR2) is found on monocytes, granulocytes, dendritic, endothelial, and epithelial cells.
Additional information: Clone REA109 displays negligible binding to Fc receptors.

Alternative names

TLR2, TIL4, Ly105

Detailed product information

Technical specifications

CloneREA109
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies, Functional-grade antibodies
Specieshuman, mouse
AntigenCD282 (TLR2)
Alternative names of antigenTLR2, TIL4, Ly105
Molecular mass of antigen [kDa]88
Entrez Gene ID7097
RRIDAB_2751976, AB_2889534, AB_2889533, AB_2661336, AB_2656979, AB_2656973, AB_2656974, AB_2656977, AB_2656978, AB_2904905, AB_2904904, AB_2904907, AB_2904906, AB_2921959, AB_2921939, AB_2752006

Resources for CD282 (TLR2) Antibody, anti-human/mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD282 (TLR2) Antibody, anti-human/mouse, REAfinity™

Publications

  1. Kang, J. Y. et al. (2009) Recognition of lipopeptide patterns by Toll-like receptor 2-Toll-like receptor 6 heterodimer. Immunity 31(6): 873-884
  2. Takeda, K. and Akira, S. (2005) Toll-like receptors in innate immunity. Int. Immunol. 17: 1-14
  3. Kirschning, C. J. and Schumann, R. R. (2002) TLR2: cellular sensor for microbial and endogenous molecular patterns. Curr Top Microbiol Immunol. 270: 121-144

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