Clone:
REA832
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
CD24A, BA-1, HAS

Extended validation for CD24 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA832
32D12+
SN3 A5-2H10+
ML5+
Cells were incubated with an excess of purified unconjugated CD24 (REA832) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD24 (REA832). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD24 (REA832). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD24 (REA832). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD24 Antibody, anti-human, REAfinity™

Overview

Clone REA832 recognizes the human CD24 antigen, which is also known as heat-stable antigen (HSA). CD24 is a glycosylphosphatidylinositol (GPI)–linked sialoglycoprotein that is anchored to the plasma membrane. CD24 has been identified to be a negative marker for breast cancer stem cells and a positive marker for ovarian or pancreatic cancer stem cells. The CD24 antibody can be used, for example, to differentiate CD44
+
CD24
breast cancer stem cells from CD24
+
expressing cells from a primary tumor sample. Furthermore, CD24 is found on follicular dendritic cells and different epithelial and hematopoietic cell types.
Additional information: Clone REA832 displays negligible binding to Fc receptors.

Alternative names

CD24A, BA-1, HAS

Detailed product information

Technical specifications

CloneREA832
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD24
Alternative names of antigenCD24A, BA-1, HAS
Molecular mass of antigen [kDa]3
Distribution of antigenB cells, endothelial cells, epithelial cells, granulocytes, Langerhans cells, monocytes, red blood cells, cancer stem cells, ES and iPS cells, leukemia cells, thymocytes, lung, ovary, pancreas, breast
Entrez Gene ID100133941
RRIDAB_2656555, AB_2656556, AB_2656557, AB_2656558, AB_2656559, AB_2656560, AB_2656561, AB_2656562, AB_2656563, AB_2656564, AB_2656565, AB_2656566, AB_2656567, AB_2656568, AB_2656569, AB_2656570, AB_2656571, AB_2811647, AB_2656554

Resources for CD24 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD24 Antibody, anti-human, REAfinity™

Publications

  1. Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
  2. Li, C. P. et al. (2007) Identification of pancreatic cancer stem cells. Cancer Res. 67: 1030-1037
  3. Gao, M. Q. et al. (2010)
    CD24
    +
    cells from hierarchically organized ovarian cancer are enriched in cancer stem cells.
    Oncogene 29: 2672-2680

Related products for
CD24 Antibody, anti-human, REAfinity™

3 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?