Clone:
REA158
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
MC, FC
Alternative names:
CCR1, CKR-1, CMKBR1, HM145, MIP1aR, SCYAR1

Extended validation for CD191 (CCR1) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA158
5F10329-
53504++
Cells were incubated with an excess of purified unconjugated CD191 (CCR1) (REA158) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD191 (CCR1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD191 (CCR1) antibodies and with a suitable counterstaining. As a control, CD191 (CCR1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD191 (CCR1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD191 (CCR1) antibodies and with a suitable counterstaining. As a control, CD191 (CCR1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD191 (CCR1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD191 (CCR1) antibodies and with a suitable counterstaining. As a control, CD191 (CCR1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD191 (CCR1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD191 (CCR1) antibodies and with a suitable counterstaining. As a control, CD191 (CCR1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD191 (CCR1) (REA158). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD191 (CCR1) (REA158). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD191 (CCR1) (REA158). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD191 (CCR1) Antibody, anti-human, REAfinity™

Overview

Clone REA158 recognizes CCR1, a chemokine receptor that binds to CC subgroup of chemokines as ligands. Chemokines are small chemoattractant protein molcules and are subdivided into families CC, CXC, or CX3C.Chemokines exert their action via binding to corresponding chemokine receptors, which are G protein–coupled receptors and share several structural features. CCR1 binds chemokines auch as CCL3, CCL5, CCL7, and CCL14 as ligands. Expression of CCR1 is found on macrophages, natural killer cells, T cells, DC, monocytes, basophils, and eosinophils, where regulated CCR1 expression can be achieved by molecules such as IL2 and TGF-β2. CCR1-ligand interaction plays role in leukocyte migration, cell differentiation/activation and immune response.
Additional information: Clone REA158 displays negligible binding to Fc receptors.      

Alternative names

CCR1, CKR-1, CMKBR1, HM145, MIP1aR, SCYAR1

Detailed product information

Technical specifications

CloneREA158
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD191 (CCR1)
Alternative names of antigenCCR1, CKR-1, CMKBR1, HM145, MIP1aR, SCYAR1
Molecular mass of antigen [kDa]41
Distribution of antigenmacrophages, monocytes, NK cells, T cells, basophils, eosinophils
Entrez Gene ID1230
RRIDAB_2655838, AB_2655839, AB_2655840, AB_2655841, AB_2655842, AB_2655843, AB_2655844, AB_2655845, AB_2801893

Resources for CD191 (CCR1) Antibody, anti-human, REAfinity™

Certificates

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References for CD191 (CCR1) Antibody, anti-human, REAfinity™

Publications

  1. Ness, T. L. et al. (2004) CCR1 and CC chemokine ligand 5 interactions exacerbate innate immune responses during sepsis. J. Immunol. 173: 6938-6948
  2. Manzo, A. et al. (2003) Role of chemokines and chemokine receptors in regulating specific leukocyte trafficking in the immune/inflammatory response. Clin. Exp. Rheumatol. 21(4): 501-508

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