The CD184 (CXCR4) MicroBead Kit has been developed for the positive selection of definitive endodermal cells differentiated from pluripotent stem cell cultures.

Data and images for CD184 (CXCR4) MicroBead Kit, human

Figures

Figure 1

CD184 (CXCR4)
+
cells were isolated from human pluripotent stem cell-derived early endoderm cells using the CD184 (CXCR4) MicroBead Kit human, an LS Column, and a MidiMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
CD184 (CXCR4)
+
cells
View details

Figure 1

CD184 (CXCR4)
+
cells were isolated from human pluripotent stem cell-derived early endoderm cells using the CD184 (CXCR4) MicroBead Kit human, an LS Column, and a MidiMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD184 (CXCR4)
+
cells were isolated from human pluripotent stem cell-derived early endoderm cells using the CD184 (CXCR4) MicroBead Kit human, an LS Column, and a MidiMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD184 (CXCR4) MicroBead Kit, human

Overview

The CD184 (CXCR4) MicroBead Kit has been developed for the positive selection of definitive endodermal cells differentiated from pluripotent stem cell cultures.

Detailed product information

Background information

CD184 is also known as CXCR4 or fusin, a 45 kDa seven transmembrane G-protein-linked chemokine receptor for SDF-1. CD184 is expressed on Sox17
+
/FoxA2
+
definitive endoderm (DE) cells that can be derived from pluripotent stem cells (PSCs) in vitro. DE differentiation is induced by high doses of Activin A in combination with Wnt and FGF pathway activation. 3-5 days after induction, DE cells can be isolated based on CD184.

Columns

LS or autoMACS
®
Columns.

References for CD184 (CXCR4) MicroBead Kit, human

Publications

  1. Davenport, C. et al. (2016) A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells J. Vis. Exp. 109: e53655

Seems like you are coming from USA!
Do you want to visit our website in your country?