Name: StraightFrom® Buffy Coat CD56 MicroBead Kit, human
Availability: InStock

Product data and images for StraightFrom
^®
Buffy Coat CD56 MicroBead Kit
, human

Figures

Figure 1

Separation of a buffy coat sample using the StraightFrom

^®

Buffy Coat CD56 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD56-PE, CD3‑APC, as well as CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Analysis gate

CD56

⁺

cells

Figure 1

Separation of a buffy coat sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Separation of a buffy coat sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for StraightFrom
^®
Buffy Coat CD56 MicroBead Kit
, human

Overview

The StraightFrom

^®

Buffy Coat CD56 MicroBead Kit has been developed for the rapid positive selection of CD56

⁺

cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS

^®

Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat.

Detailed product information

Background information

The CD56 antigen is expressed by most NK cells and a small subset of T cells. Upon activation of NK cells, the surface expression of CD56 is increased.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom

^®

Buffy Coat CD56 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS

^®

Separator. After removing the column from the magnetic field, the magnetically retained CD56

⁺

cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD56

⁺

cells are well suited for further flow cytometric, functional, or molecular analysis.

Resources for StraightFrom
^®
Buffy Coat CD56 MicroBead Kit
, human

Documents and Protocols

Brochures/posters

StraightFrom® MicroBeads - Cell isolation directly from blood products

Brochures/posters

Everything you need for your natural killer cell research

Scientific posters

Isolation of functional cells straight from buffy coat, LRSC, and Leukopak®

App notes/customer reports

Automated isolation of CD56⁺ natural killer cells and antibody-dependent cellular cytotoxicity.

Data and images

Data and images for StraightFrom
^®
Buffy Coat CD56 MicroBead Kit
, human

Figures

Figure 1

Separation of a buffy coat sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Analysis gate

CD56

⁺

cells

Figure 1

Separation of a buffy coat sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Separation of a buffy coat sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for StraightFrom
^®
Buffy Coat CD56 MicroBead Kit
, human

Overview

The StraightFrom

^®

Buffy Coat CD56 MicroBead Kit has been developed for the rapid positive selection of CD56

⁺

cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS

^®

Detailed product information

Background information

The CD56 antigen is expressed by most NK cells and a small subset of T cells. Upon activation of NK cells, the surface expression of CD56 is increased.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom

^®

Buffy Coat CD56 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS

^®

Separator. After removing the column from the magnetic field, the magnetically retained CD56

⁺

cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD56

⁺

cells are well suited for further flow cytometric, functional, or molecular analysis.

Resources

Resources for StraightFrom
^®
Buffy Coat CD56 MicroBead Kit
, human

Documents and Protocols

Brochures/posters

Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

StraightFrom® Buffy Coat CD56 MicroBead Kit, human

StraightFrom® Buffy Coat CD56 MicroBead Kit, human

Product data and images for StraightFrom® Buffy Coat CD56 MicroBead Kit, human

Figures

Figure 1