Background information
Krüppel-like factor 4 (Klf4) is a zinc finger transcription factor that plays an important role in maintaining pluripotent stem cells and preventing their differentiation. Klf4 regulates cell lineage differentiation during development and maintains epithelial homeostasis in the intestine. Exogenous expression of Klf4 in combination with other transcription factors has been used to generate induced pluripotent stem cells.
Detailed procedure
For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.
Quality description
StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
