The Neonatal Cardiac Endothelial Cell Isolation Kit, rat has been developed for the two-step isolation of vital cardiac endothelial cells from neonatal rat hearts (P0-P3). Enriched cardiac endothelial cells are fully functional and can be used for various downstream applications.

Data and images for Neonatal Cardiac Endothelial Cell Isolation Kit, rat

Figures

Figure 1

Cardiac endothelial cells were isolated from P1 Wistar rat hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD column with MidiMACS Separator and one MS column with MiniMACS Separator. The cells were fluorescently stained with CD90.1-PE (#130-102-636) and CD31-APC (#130-105-937 ) and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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Figure 1

Cardiac endothelial cells were isolated from P1 Wistar rat hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD column with MidiMACS Separator and one MS column with MiniMACS Separator. The cells were fluorescently stained with CD90.1-PE (#130-102-636) and CD31-APC (#130-105-937 ) and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Cardiac endothelial cells were isolated from P1 Wistar rat hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD column with MidiMACS Separator and one MS column with MiniMACS Separator. The cells were fluorescently stained with CD90.1-PE (#130-102-636) and CD31-APC (#130-105-937 ) and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Neonatal Cardiac Endothelial Cell Isolation Kit, rat

Overview

The Neonatal Cardiac Endothelial Cell Isolation Kit, rat has been developed for the two-step isolation of vital cardiac endothelial cells from neonatal rat hearts (P0-P3). Enriched cardiac endothelial cells are fully functional and can be used for various downstream applications.

Detailed product information

Background information

Besides cardiomyocytes and fibroblasts, endothelial cells represent the third most frequent cell population in the neonatal mouse heart. Endothelial cells form the inner layer of blood vessels and play a key role in vascular development, homeostasis and remodeling. The Neonatal Cardiac Endothelial Cell Isolation Kit, rat has been designed for enrichment of cardiac endothelial cells from dissociated neonatal rat hearts. The isolation of cardiac endothelial cells has successfully been tested using neonatal rat hearts from postnatal day 0 to day 3 (P0-P3). For optimal use, the Neonatal Cardiac Endothelial Cell Isolation Kit should be used in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373).

Columns

For the first magnetic separation (depletion): LD Columns. For the second magnetic separation (positive selection): MS Columns.

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