The MACSQuant Tyto Cartridge HS has been specifically designed to facilitate never-before-seen
high-speed
cell sorting protocols on the MACSQuant Tyto Cell Sorter, shortening the required sample processing time significantly. In addition, due to its cutting-edge microfluidic pathway, the MACSQuant Tyto Cartridge HS also features inertial cell focusing to improve sorting performance.
As in the standard MACSQuant Tyto Cartridge, the patented microchip technology of the MACSQuant Tyto Cartridge HS enables fluorescence-based and aerosol-free cell sorting with the world’s fastest mechanical sort valve (30,000 valve

Data and images for
MACSQuant
®
Tyto
®
Cartridges HS

Figures

Figure 1

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The sorting mechanism of the MACSQuant Tyto Cell Sorter. Coming from the input chamber, cells enter the microchip through a microchannel where they are interrogated by three lasers. Before entering the microchannel, potential cell aggregates are held back by a filter system, guaranteeing a smooth sorting process.
When a target cell (magenta) is identified, a magnetic pulse coming from the solenoid opens the microvalve, which then redirects the target cell into the positive collection chamber. In the default state, the valve is closed allowing non-selected cells (blue and orange) to flow through into the negative collection chamber.

Figure 1

The sorting mechanism of the MACSQuant Tyto Cell Sorter. Coming from the input chamber, cells enter the microchip through a microchannel where they are interrogated by three lasers. Before entering the microchannel, potential cell aggregates are held back by a filter system, guaranteeing a smooth sorting process.
When a target cell (magenta) is identified, a magnetic pulse coming from the solenoid opens the microvalve, which then redirects the target cell into the positive collection chamber. In the default state, the valve is closed allowing non-selected cells (blue and orange) to flow through into the negative collection chamber.

Figure 2

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The MACSQuant Tyto Cartridge HS, side view

Figure 2

The MACSQuant Tyto Cartridge HS, side view

Figure 3

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The MACSQuant Tyto Cartridge HS, bottom view

Figure 3

The MACSQuant Tyto Cartridge HS, bottom view

Figure 4

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The MACSQuant Tyto Cartridge HS, bottom view showing the inertial focusing interposer, including the mounted microchip

Figure 4

The MACSQuant Tyto Cartridge HS, bottom view showing the inertial focusing interposer, including the mounted microchip

Specifications for
MACSQuant
®
Tyto
®
Cartridges HS

Overview

The MACSQuant Tyto Cartridge HS has been specifically designed to facilitate never-before-seen
high-speed
cell sorting protocols on the MACSQuant Tyto Cell Sorter, shortening the required sample processing time significantly. In addition, due to its cutting-edge microfluidic pathway, the MACSQuant Tyto Cartridge HS also features inertial cell focusing to improve sorting performance.
As in the standard MACSQuant Tyto Cartridge, the patented microchip technology of the MACSQuant Tyto Cartridge HS enables fluorescence-based and aerosol-free cell sorting with the world’s fastest mechanical sort valve (30,000 valve actuations per second) in a fully closed environment, providing complete operator safety.
The MACSQuant Tyto Cartridge HS is a single-use disposable that diminishes any risk of sample cross-contamination. Both positive and negative sorted fractions remain in the MACSQuant Tyto Cartridge HS after the actual sort, allowing full retrieval of all cells within the sample.
ATTENTION: To be able to run the MACSQuant® Tyto® Cartridge HS on your MACSQuant Tyto Cell Sorter, MACSQuantify™ Tyto Software version 0.5.1 or higher is required! For further information, please contact technical support.

Detailed product information

Background information

The MACSQuant Tyto Cartridge HS consists of three compartments that can each be accessed from above through individual, aseptic Luer-Locks: the input chamber, the positive collection chamber, and the negative collection chamber.
Cells suspended in the specially formulated MACSQuant Tyto Running Buffer are loaded into the input chamber of the MACSQuant Tyto Cartridge. The input chamber contains an adjustable propeller, which keeps cells in suspension during the sort.
After loading the cartridge into the instrument, filtered air is applied to the input chamber through a pressure inlet. Driven by moderate air pressure, fluorescently labeled cells flow from the input chamber through an inertial focusing microchannel into the microchip. Within the microchip, cells are interrogated by three lasers. Fluorescent and scatter light signatures are used to select target cells, which are then redirected by a sort valve located within the sorting channel.
To gently redirect a target cell into the positive collection chamber, a magnetic field is applied to the microchip. This triggers the sorting valve to open and therefore change the flow of the target cell towards the positive collection chamber.
After a target cell has passed through, a silicon spring returns the valve into its original position. Unlike during conventional droplet sorting procedures, the cells do not experience very high pressures of up to 70 psi, and therefore are not as strongly decompressed as in these systems. In addition, no charge is applied, and the cells experience only very mild shear forces, resulting in a high viability and functionality of the sorted cells.

Technical data

Video

MACSQuant Tyto: Microchip-based cell sorting in a fully closed cartridge system

Find out more about cell sorting on the MACSQuant Tyto: It’s simple, it’s gentle, it’s sterile. This video explains step by step how the unique sorting mechanism within the MACSQuant Tyto Cartridge works.

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