MACSprep™ Chimerism CD3 MicroBeads have been developed for the positive selection of CD3
+
cells directly from anticoagulated whole blood by using the autoMACS
®
Pro Separator, the MultiMACS Cell24 Separator Plus, or the Whole Blood Column Kit. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis, as well as no washing step after labeling.

Data and images for MACSprep™ Chimerism CD3 MicroBeads, human

Figures

Figure 1

Separation of a whole blood sample using the MACSprep™ Chimerism CD3 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD3-PE, CD14‑APC, as well as CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
After separation
View details

Figure 1

Separation of a whole blood sample using the MACSprep™ Chimerism CD3 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD3-PE, CD14‑APC, as well as CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Separation of a whole blood sample using the MACSprep™ Chimerism CD3 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD3-PE, CD14‑APC, as well as CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for MACSprep™ Chimerism CD3 MicroBeads, human

Overview

MACSprep™ Chimerism CD3 MicroBeads have been developed for the positive selection of CD3
+
cells directly from anticoagulated whole blood by using the autoMACS
®
Pro Separator, the MultiMACS Cell24 Separator Plus, or the Whole Blood Column Kit. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis, as well as no washing step after labeling.

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