Clone:
REA1016
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
2F1-Ag, MAFA, MAFA-L

Extended validation for KLRG1 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1016
2F1/KLRG1++
2F1++
Cells were incubated with an excess of purified unconjugated KLRG1 (REA1016) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using KLRG1 (REA1016). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using KLRG1 (REA1016). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using KLRG1 (REA1016). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for KLRG1 Antibody, anti-mouse, REAfinity™

Overview

Clone REA1016 recognizes the mouse killer cell lectin-like receptor G1 (KLRG1) antigen. KLRG1 is a homodimeric type II transmembrane glycoprotein of 30–38 kDa subunits. The protein is the mouse homolog of the rat mast cell function–associated antigen (MAFA) and is expressed on a subset of NK cells and T cells. Several members of the cadherin family can interact with KLRG1 leading to inhibition of NK cell and T cell effector functions.
Additional information: Clone REA1016 displays negligible binding to Fc receptors.

Alternative names

2F1-Ag, MAFA, MAFA-L

Detailed product information

Technical specifications

CloneREA1016
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenKLRG1
Alternative names of antigen2F1-Ag, MAFA, MAFA-L
Molecular mass of antigen [kDa]21
Distribution of antigenNK cells, T cells
Entrez Gene ID50928
RRIDAB_2727832, AB_2727823, AB_2727833, AB_2727824, AB_2727834, AB_2727825, AB_2727835, AB_2727826, AB_2727836, AB_2727827

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