Clone:
JES6-5H4
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
rat IgG2bκ
Applications:
FA, ICFC, MICS, IF, IHC

Extended validation for IL-2 Antibody, anti-mouse

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for IL-2 Antibody, anti-mouse

Overview

Interleukin 2 (IL-2), mainly produced by activated CD4
+
T cells, is a central regulator of the immune response. It induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, monocytes. and oligodendrocytes. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance, and anti-inflammatory reactions.

Detailed product information

Technical specifications

CloneJES6-5H4
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies, Functional-grade antibodies
Speciesmouse
AntigenIL-2
Molecular mass of antigen [kDa]17
Entrez Gene ID16183
RRIDAB_2660009, AB_2660010, AB_10829301

Resources for IL-2 Antibody, anti-mouse

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Related products for
IL-2 Antibody, anti-mouse

7 products available | view all

Seems like you are coming from USA!
Do you want to visit our website in your country?