The GM-CSF Secretion Assay - Detection Kit was developed for sensitive detection of human GM-CSF-secreting cells.

Data and images for GM-CSF Secretion Assay - Detection Kit (PE), human

Figures

Figure 1

PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained according to secretion of GM-CSF using the GM-CSF Secretion Assay - Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively.
Stimulated sample
Unstimulated control
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Figure 1

PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained according to secretion of GM-CSF using the GM-CSF Secretion Assay - Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively.
View details

Figure 1

PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained according to secretion of GM-CSF using the GM-CSF Secretion Assay - Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively.

Specifications for GM-CSF Secretion Assay - Detection Kit (PE), human

Overview

The GM-CSF Secretion Assay - Detection Kit was developed for sensitive detection of human GM-CSF-secreting cells.

Detailed product information

Background information

Granulocyte macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor that is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Downstream applications

The GM-CSF Secretion Assay can be used for the detection of viable GM-CSF-secreting leukocytes. Antigen-specific T cell immunity, e.g. in infection, autoimmunity and allergy, can be monitored and analyzed with this detection kit, as well as enumeration and phenotypic or functional characterization of GM-CSF-secreting cells. The GM-CSF Secretion Assay – Detection Kit (PE) can be combined with a second Detection Kit (FITC or APC) to analyze co-expressed cytokines.

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