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Co-cultures of mouse embryonic stem (mES) cells and mouse embryonic fibroblast (mEF) cells were stained with Anti-Feeder Cells antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. mEF cells could be clearly discriminated from unstained mES cells based on the Anti-Feeder Cells antibody staining. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Specifications for Feeder Cells Antibody, anti-mouse
Overview
The most prevalent cultivation method for maintenance of mouse and human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells is co-cultivation on primary mouse embryonic fibroblast (mEF) feeder cell layers. Feeder cell layers of NIH3T3 fibroblasts or stromal derived OP9 cells are also frequently used in co-culture systems: NIH3T3 fibroblasts support primary keratinocyte cultures, OP9 cells are used for hematopoietic induction of ES cells.
Specifications for Feeder Cells Antibody, anti-mouse
Overview
The most prevalent cultivation method for maintenance of mouse and human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells is co-cultivation on primary mouse embryonic fibroblast (mEF) feeder cell layers. Feeder cell layers of NIH3T3 fibroblasts or stromal derived OP9 cells are also frequently used in co-culture systems: NIH3T3 fibroblasts support primary keratinocyte cultures, OP9 cells are used for hematopoietic induction of ES cells.
