CD8
⁺
CD45RA
⁺
Effector T Cell Isolation Kit
, human

Name: CD8+CD45RA+ Effector T Cell Isolation Kit, human
Availability: InStock

CD8
⁺
CD45RA
⁺
Effector T Cell Isolation Kit
, human

The CD8

⁺

CD45RA

⁺

Effector T Cell Isolation Kit has been developed for the positive selection of CD8

⁺

CD45RA

⁺

effector T cells from human PBMCs.

Product data and images for CD8
⁺
CD45RA
⁺
Effector T Cell Isolation Kit
, human

Figures

Figure 1

CD8

⁺

CD45RA

⁺

effector T cells were isolated from human PBMCs using the CD8

⁺

CD45RA

⁺

Effector T Cell Isolation Kit, an LD Column, an MS Column, a MidiMACS™ Separator, and a MiniMACS™ Separator.

PBMCs before separation

Gated on viable CD8 ⁺ CD56 ^– cells.
View details CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit , human Figure 1 CD8 ⁺ CD45RA ⁺ effector T cells were isolated from human PBMCs using the CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit, an LD Column, an MS Column, a MidiMACS™ Separator, and a MiniMACS™ Separator.	View details CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit , human Figure 1 CD8 ⁺ CD45RA ⁺ effector T cells were isolated from human PBMCs using the CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit, an LD Column, an MS Column, a MidiMACS™ Separator, and a MiniMACS™ Separator.
Pre-enriched CD8⁺CD45RA⁺ effector T cells

Gated on viable CD8 ⁺ CD56 ^– cells.
View details CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit , human Figure 1 CD8 ⁺ CD45RA ⁺ effector T cells were isolated from human PBMCs using the CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit, an LD Column, an MS Column, a MidiMACS™ Separator, and a MiniMACS™ Separator.	View details CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit , human Figure 1 CD8 ⁺ CD45RA ⁺ effector T cells were isolated from human PBMCs using the CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit, an LD Column, an MS Column, a MidiMACS™ Separator, and a MiniMACS™ Separator.
Isolated CD8⁺CD45RA⁺ effector T cells

Gated on viable CD8 ⁺ CD56 ^– cells.
View details CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit , human Figure 1 CD8 ⁺ CD45RA ⁺ effector T cells were isolated from human PBMCs using the CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit, an LD Column, an MS Column, a MidiMACS™ Separator, and a MiniMACS™ Separator.	View details CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit , human Figure 1 CD8 ⁺ CD45RA ⁺ effector T cells were isolated from human PBMCs using the CD8 ⁺ CD45RA ⁺ Effector T Cell Isolation Kit, an LD Column, an MS Column, a MidiMACS™ Separator, and a MiniMACS™ Separator.

Specifications for CD8
⁺
CD45RA
⁺
Effector T Cell Isolation Kit
, human

Overview

The CD8

⁺

CD45RA

⁺

Effector T Cell Isolation Kit has been developed for the positive selection of CD8

⁺

CD45RA

⁺

effector T cells from human PBMCs.

Detailed product information

Background information

The CD8

⁺

CD45RA

⁺

Effector T Cell Isolation Kit is ideal for the isolation of cytotoxic T cells that express high levels of perforin and granzyme B

¹

. Isolated cells can be analyzed for interactions with other cell types and degranulation or secretion of effector molecules.

Detailed separation procedure

The isolation of CD8

⁺

CD45RA

⁺

effector T cells is performed in a two-step procedure. First, non-target cells are indirectly magnetically labeled for depletion with biotin-conjugated antibodies against CD45RO and CD56 and an APC-conjugated antibody against CD197 (CCR7), as well as Anti-APC MicroBeads and Anti-Biotin MicroBeads. In the second step, the CD8

⁺

CD45RA

⁺

effector T cells are directly labeled with CD8 MicroBeads and isolated by positive selection from the pre-enriched cell fraction.

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

References for CD8
⁺
CD45RA
⁺
Effector T Cell Isolation Kit
, human

Publications

Hamann, D. et al. (1997)
Phenotypic and functional separation of memory and effector human CD8
⁺
T cells.
J. Exp. Med. 186: 1407-1418

Data and images