CD43 MicroBeads, human

CD43 MicroBeads, human

CD43 MicroBeads were developed for the isolation of untouched resting human B cells from PBMCs or lymphoid tissues, based on the magnetic depletion of cells that express the CD43 antigen.

Data and images
Specifications

Product data and images for CD43 MicroBeads, human

Figures

Figure 1

Isolation of untouched CD43

^–

B cells from PBMCs by depletion with CD43 MicroBeads using an LS Column and a MidiMACS™ Separator.

Unseparated fraction	CD19 ⁺ CD43 ^– B cells
View details CD43 MicroBeads, human Figure 1 Isolation of untouched CD43 ^– B cells from PBMCs by depletion with CD43 MicroBeads using an LS Column and a MidiMACS™ Separator.	View details CD43 MicroBeads, human Figure 1 Isolation of untouched CD43 ^– B cells from PBMCs by depletion with CD43 MicroBeads using an LS Column and a MidiMACS™ Separator.
CD19 ⁺ CD43 ^– B cells
View details CD43 MicroBeads, human Figure 1 Isolation of untouched CD43 ^– B cells from PBMCs by depletion with CD43 MicroBeads using an LS Column and a MidiMACS™ Separator.

Specifications for CD43 MicroBeads, human

Overview

CD43 MicroBeads were developed for the isolation of untouched resting human B cells from PBMCs or lymphoid tissues, based on the magnetic depletion of cells that express the CD43 antigen.

Detailed product information

Background information

CD43 (leukosialin, sialophorin) is a cell surface glycoprotein that is involved in adhesion, anti-adhesion, and signal transduction processes. The CD43 antigen is expressed on most leukocytes, i.e., T cells, NK cells, granulocytes, monocytes, and macrophages, on hematopoietic stem cells, and on platelets, but not on erythrocytes. Among B cells, CD43 is expressed on activated B cells and plasma cells but not on resting B cells, e.g., naive B cells. In bone marrow, CD43 is found on pro-B cells but is down-regulated during transition to the pre-B cell stage.

Downstream applications

Resting B cells, isolated with CD43 MicroBeads, can be used for studies on signal transduction processes, e.g., B cell activation or differentiation, and related molecular processes. The magnetically unlabeled CD43

^–

cells may also be used for further sorting of B cell subsets using MACS® Technology.

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Data and images