The CD3ε MicroBead Kit was developed for depletion of mouse T cells from single-cell supensions from spleen, lymph node, and thymus.

Data and images for CD3ε MicroBead Kit, mouse

Figures

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD3ε cells were depleted from this suspension using the CD3ε MicroBead Kit, an LD Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD45R (B220)-FITC and Anti-Biotin-PE, and analyzed using the MACSQuant® Analyzer.
PBMCs before separation
CD3ε
-
cells
View details

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD3ε cells were depleted from this suspension using the CD3ε MicroBead Kit, an LD Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD45R (B220)-FITC and Anti-Biotin-PE, and analyzed using the MACSQuant® Analyzer.
View details

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD3ε cells were depleted from this suspension using the CD3ε MicroBead Kit, an LD Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD45R (B220)-FITC and Anti-Biotin-PE, and analyzed using the MACSQuant® Analyzer.

Specifications for CD3ε MicroBead Kit, mouse

Overview

The CD3ε MicroBead Kit was developed for depletion of mouse T cells from single-cell supensions from spleen, lymph node, and thymus.

Detailed product information

Background information

CD3ε is a 20 kDa transmembrane protein and subunit of the TCR complex. Along with the γ and δ subunits of CD3, the ε chain is required for assembly and surface expression of the TCR complex. CD3 is expressed on thymocytes, mature T lymphocytes, and natural killer T (NKT) cells. With the CD3ε MicroBead Kit, mouse T lymphocytes and NKT cells can be depleted from various tissues including spleen, thymus, or lymph nodes.

Columns

LD or autoMACS
®
Columns.

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