Clone:
REA107
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
CXCR4, Cmkar4, LESTR, PB-CKR, PBSF/SDF-1, Sdf1r, b2b220Clo, Fusin, SDF-1 receptor

Extended validation for CD184 (CXCR4) Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA107
L276F12-
2B11++
Cells were incubated with an excess of purified unconjugated CD184 (CXCR4) (REA107) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Thymocytes from BALB/c mice were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Thymocytes from BALB/c mice were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Thymocytes from BALB/c mice were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Thymocytes from BALB/c mice were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD184 (CXCR4). Thymocytes from BALB/c mice were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD184 (CXCR4) (REA107). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD184 (CXCR4) (REA107). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD184 (CXCR4) (REA107). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD184 (CXCR4) Antibody, anti-mouse, REAfinity™

Overview

Clone REA107 reacts with CD184, a receptor for C-X-C chemokine CXCL12/SDF-1. CD184 is a seven membrane spanning receptor and belongs to the family of G-protein coupled chemokine receptors. Expression of CD184 is found in tissues including spleen, thymus, bone marrow, lymph nodes, and at lower levels in small intestine, kidney, and stomach. Diverse functions of CD184 include promoting survival and proliferation of hematopoietic stem cells, inducing apoptosis in T cells, regulating osteoclastogenesis, B cell development, vascularization of the gastrointestinal tract, germ cell migration/survival, and cerebellar development. CD184 also serves as a co-receptor for entry into target cells by HIV-1 X4 and interacts with extracellular ubiquitin.
Additional information: Clone REA107 displays negligible binding to Fc receptors.

Alternative names

CXCR4, Cmkar4, LESTR, PB-CKR, PBSF/SDF-1, Sdf1r, b2b220Clo, Fusin, SDF-1 receptor

Detailed product information

Technical specifications

CloneREA107
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD184 (CXCR4)
Alternative names of antigenCXCR4, Cmkar4, LESTR, PB-CKR, PBSF/SDF-1, Sdf1r, b2b220Clo, Fusin, SDF-1 receptor
Molecular mass of antigen [kDa]40
Distribution of antigenB cells, T cells, stem cells, hematopoietic stem and progenitor cells
Entrez Gene ID12767
RRIDAB_2802020, AB_2819677, AB_2655760, AB_2655761, AB_2655764, AB_2655765, AB_2733781

References for CD184 (CXCR4) Antibody, anti-mouse, REAfinity™

Publications

  1. Drummond, R. A. et al. (2014)
    Cutting edge: Failure of antigen-specific CD4
    +
    T cell recruitment to the kidney during systemic candidiasis.
    J. Immunol. 193(11): 5381-5385
  2. Förster, R. et al. (1998) Intracellular and surface expression of the HIV-1 coreceptor CXCR4/Fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J. Immunol. 160: 1522 -1531
  3. Tachibana, K. et al. (1998) The chemokine receptor CXCR4 is essential for vascularization of the gastrointestinal tract. Nature 393: 591-594
  4. Zou, Y.-R. et al. (1998) Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development. Nature 393: 595-599
  5. Wegner, S. A. et al. (1998) Genomic organization and functional characterization of the chemokine receptor CXCR4, a major entry co-receptor for human immunodeficiency virus type 1. J. Biol. Chem. 273(8): 4754-4760

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