Specifications for CD154 Antibody, anti-mouse, REAfinity™
Overview
Clone REA785 specifically recognizes the mouse CD154 antigen, a 39 kDa transmembrane glycoprotein also known as CD40L, gp39, T-BAM, TRAP, or Ly-62. CD154 is transiently up-regulated on activated CD4
+ T cells and plays an important role as a costimulatory molecule in T cell/antigen–presenting cell interactions through ligation of CD40. Due to its transient expression within hours after activation, CD154 can be used as a marker for activated antigen-specific CD4
+ T cells. Adding a CD40-blocking antibody during the stimulation of cell suspensions prevents the down-regulation of CD154. Blocking of CD40 is not required for intracellular detection of CD154 expression or if a pure population of enriched T cells is used.
Additional information: Clone REA785 displays negligible binding to Fc receptors.
Alternative names
CD40LG, CD40L, HIGM1, IGM, IMD3, Ly-62, T-BAM, TRAP, TNFSF5, gp39Detailed product information
Technical specifications
| Clone | REA785 |
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| Clonality | monoclonal |
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| Isotype | recombinant human IgG1 |
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| Isotype control | REA Control Antibody, human IgG1 |
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| Host | human cell line |
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| Type of antibody | Primary antibodies, Recombinant antibodies |
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| Species | mouse |
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| Antigen | CD154 |
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| Alternative names of antigen | CD40LG, CD40L, HIGM1, IGM, IMD3, Ly-62, T-BAM, TRAP, TNFSF5, gp39 |
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| Molecular mass of antigen [kDa] | 29 |
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| Distribution of antigen | dendritic cells, lymphocytes, macrophages, monocytes, T cells, NK cells, mast cells, basophils |
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| Entrez Gene ID | 21947 |
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| RRID | AB_2655274, AB_2655275, AB_2655276, AB_2655277, AB_2655278, AB_2655279, AB_2655280, AB_2922143, AB_2655273 |
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