The CD137 MicroBead Kit was developed for the positive selection of human CD137
+
cells from PBMCs.

Data and images for CD137 MicroBead Kit, human

Figures

Figure 1

PBMCs of a CMV
+
donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137
+
T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.
Stimulated sample
Unseparated fraction
After separation
View details

Figure 1

PBMCs of a CMV
+
donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137
+
T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.
View details

Figure 1

PBMCs of a CMV
+
donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137
+
T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.
Unstimulated control
Unseparated fraction
After separation
View details

Figure 1

PBMCs of a CMV
+
donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137
+
T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.
View details

Figure 1

PBMCs of a CMV
+
donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137
+
T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.

Specifications for CD137 MicroBead Kit, human

Overview

The CD137 MicroBead Kit was developed for the positive selection of human CD137
+
cells from PBMCs.

Detailed product information

Background information

CD137-PE recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4
+
and CD8
+
T cells, activated B cells, and on natural killer cells, but can also be found on resting monocytes and dendritic cells. As a costimulatory molecule CD137 is involved in the activation and survival of CD4
+
or CD8
+
T cells, and NK cells.
Recently, CD137 has been described to be a suitable marker for antigen-specific activation of human CD8
+
T cells, as CD137 is not expressed on resting CD8
+
T cells and its expression is reliably induced after 24 hours of stimulation.
1
CD137
+
cells are indirectly magnetically labeled with CD137-PE and Anti-PE MicroBeads. For the isolation of activated antigen-specific CD8
+
T cells, the CD137 MicroBead Kit can be combined with the CD8
+
T Cell Isolation Kit.

Downstream applications

In a previous study, CD137-expressing T cells were targeted for selective depletion of alloreactive T cells from antileukemic and antitumor donor T cell lines.
2
The CD137 MicroBead Kit was developed for positive selection of CD137
+
cells for phenotypical and functional characterization of activated CD137
+
T cells.

Columns

MS, LS, or autoMACS
®
Columns.

References for CD137 MicroBead Kit, human

Publications

  1. Schmied, S. et al. (2015)
    Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4
    +
    and CD8
    +
    memory formation.
    Immunology 145(4): 558-569
  2. Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928
  3. Wolfl, M. et al. (2007)
    Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8
    +
    T cells responding to antigen without requiring knowledge of epitope specificities.
    Blood 110: 201-210
  4. Wehler, T. C. et al. (2007) Targeting the activation-induced antigen CD137 can selectively deplete alloreactive T cells from antileukemic and antitumor donor T-cell lines. Blood 109: 365-373

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