Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human
Applications:
FC

Data and images for Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human

Figures

Figure 1

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Dendritic cell subset–specific antigens.

Figure 1

Dendritic cell subset–specific antigens.

Figure 2

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Staining procedure of the Blood Dendritic Cell Enumeration Kit.

Figure 2

Staining procedure of the Blood Dendritic Cell Enumeration Kit.

Specifications for Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human

Overview

The Blood Dendritic Cell Enumeration Kit was developed for easy identification and enumeration of dendritic cells (DCs) and DC subsets in whole blood or PBMCs by flow cytometry. All reagents required for the analysis are included in the kit.
Applications of the kit include monitoring of peripheral blood DC frequency and number, for example, after hematopoietic stem cell mobilization
3
, or in the course of pathogenic infections, for example by HIV.
4
Furthermore, DC numbers were determined in patients with chronic graft versus host disease
5
, inflammatory bowel disease
6
, psoriatic arthritis and rheumatoid arthritis
10
, under long-term immunosuppression
8
, lymphoproliferative disease of granular lymphocytes
9
, or in patients with hepatocellular carcinoma, in order to analyze the immunosuppressive role of IL-10 on circulating dendritic cells
7
.

Detailed product information

Background information

BDC Phenotyping Cocktail, anti-human containing:
  • CD1c (BDCA-1) Antibody, anti-human, PE (clone: AD5-8E7, isotype: mouse IgG2a)
  • CD303 (BDCA-2) Antibody, anti-human, FITC (clone: AC144, isotype: mouse IgG1)
  • CD141 (BDCA-3) Antibody, anti-human, APC (clone: AD5-14H12, isotype: mouse IgG1)
  • CD20 Antibody, anti-human, PerCP (clone: LT20, isotype: mouse IgG1κ)
  • CD14 Antibody, anti-human, PerCP (clone: TÜK4, isotype: mouse IgG2aκ)
BDC Control Cocktail, anti-human containing:
  • Isotype Control Antibody, mouse IgG2a, PE (clone: S43.10, isotype: mouse IgG2a)
  • Cytokeratin Antibody, anti-human, FITC (clone: CK3-6H5, isotype: mouse IgG1κ)
  • Biotin Antibody, APC (clone: Bio3-18E7, isotype: mouse IgG1)
  • CD20 Antibody, anti-human, PerCP (clone: LT20, isotype: mouse IgG1κ)
  • CD14 Antibody, anti-human, PerCP (clone: TÜK4, isotype: mouse IgG2aκ)
Additionally
  • Red Blood Cell Lysis Solution (10×)
  • Dead Cell Discriminator
  • Discriminator Stop Reagent
  • Fix Solution
Instrument details
Flow cytometer equipped with a red (640 nm) and a blue (488 nm) laser.

Applications

Identification and enumeration of DCs and DC subsets in whole blood or peripheral blood mononuclear cells (PBMCs).

Resources for Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human

Documents and Protocols

Scientific posters

Blood DC preparations generated using automated CliniMACS Prodigy® CD1c/CD304 enrichment and activation system efficiently activate CD8+ antigen-specific T cells

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human

Publications

  1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol 165: 6037-6046
  2. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 36: 1133-1148
  3. Arpinati, M. et al. (2002) Use of anti-BDCA-2 antibody for detection of dendritic cell type-2 (DC2) in allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant. 29: 887-891
  4. Chehimi, J. et al. (2002) Persistent decreases in blood plasmacytoid dendritic cell number and function despite effective highly active antiretroviral therapy and increased blood myeloid dendritic cells in HIV-infected individuals. J Immunol 168: 4769-4801
  5. Clark, F. J. et al. (2003) Origin and subset distribution of peripheral blood dendritic cells in patients with chronic graft-versus-host disease. Transplantation 75: 221-225
  6. Baumgart, D. C. et al. (2005) Patients with active inflammatory bowel disease lack immature peripheral blood plasmacytoid and myeloid dendritic cells. Gut 54: 228-236
  7. Hackstein, H. et al. (2005) Dendritic cell deficiency in the blood of kidney transplant patients on long-term immunosuppression: results of a prospective matched-cohort study. Am. J. Transplant. 5: 2945-2953
  8. Zambello, R. et al. (2005) Phenotypic and functional analyses of dendritic cells in patients with lymphoproliferative disease of granular lymphocytes (LDGL). Blood 106: 3926-3931
  9. Jongbloed, S. L. et al. (2006) Enumeration and phenotypical analysis of distinct dendritic cell subsets in psoriatic arthritis and rheumatoid arthritis. Arthritis Res. Ther. 8: R15

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