The Basophil Isolation Kit II is an indirect magnetic labeling system for the negative isolation of basophils from PBMCs.

Data and images for Basophil Isolation Kit II, human

Figures

Figure 1

Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c
+
cells or CD123
bright
CD303
cells because CD123 is expressed at high levels on both basophils and CD303
+
plasmacytoid dendritic cells.
Unseparated fraction
A:
B:
View details

Figure 1

Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c
+
cells or CD123
bright
CD303
cells because CD123 is expressed at high levels on both basophils and CD303
+
plasmacytoid dendritic cells.
View details

Figure 1

Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c
+
cells or CD123
bright
CD303
cells because CD123 is expressed at high levels on both basophils and CD303
+
plasmacytoid dendritic cells.
Isolated basophils
A:
B:
View details

Figure 1

Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c
+
cells or CD123
bright
CD303
cells because CD123 is expressed at high levels on both basophils and CD303
+
plasmacytoid dendritic cells.
View details

Figure 1

Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c
+
cells or CD123
bright
CD303
cells because CD123 is expressed at high levels on both basophils and CD303
+
plasmacytoid dendritic cells.

Figure 2

View details
Basophils isolated with MACS
®
Technology using the Basophil Isolation Kit II. (Courtesy of Dr. Karin Haisch, Borstel, Germany.)

Figure 2

Basophils isolated with MACS
®
Technology using the Basophil Isolation Kit II. (Courtesy of Dr. Karin Haisch, Borstel, Germany.)

Specifications for Basophil Isolation Kit II, human

Overview

The Basophil Isolation Kit II is an indirect magnetic labeling system for the negative isolation of basophils from PBMCs.

Detailed product information

Detailed separation procedure

Non-basophils (i.e. T cells, NK cells, B cells, monocytes, dendritic cells, erythroid cells, platelets, neutrophils, and eosinophils) are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure basophils is achieved by depletion of the magnetically labeled cells.

Downstream applications

Isolated basophils have been used for phenotypical characterization
3
, for histamine, leukotriene, and cytokine
1, 4, 5, 6
release assays, for molecular studies
1, 2, 5
, protein analysis
4, 5
, and other applications.

Columns

LS, XS, or autoMACS
®
Columns.

References for Basophil Isolation Kit II, human

Publications

  1. Shiratori, I. et al. (2005) Down-regulation of basophil function by human CD200 and human herpesvirus-8 CD200. J. Immunol. 175: 4441-4449
  2. Nishiyama, C. et al. (2004) Polymorphisms in the Fc epsilon RI beta promoter region affecting transcription activity: a possible promoter-dependent mechanism for association between Fc epsilon RI beta and atopy. J. Immunol. 173: 6458-6464
  3. de Paulis, A. et al. (2004) Urokinase induces basophil chemotaxis through a urokinase receptor epitope that is an endogenous ligand for formyl peptide receptor-like 1 and -like 2. J. Immunol. 173: 5739-5748
  4. Gibbs, B. F. et al. (2002) Regulation of mediator secretion in human basophils by p38 mitogen-activated protein kinase: phosphorylation is sensitive to the effects of phosphatidylinositol 3-kinase inhibitors and calcium mobilization. J. Leukoc. Biol. 72: 391-400
  5. Lavens-Phillips, S.E. et al. (2000) The tyrosine kinases p53/56lyn and p72syk are differentially expressed at the protein level but not at the messenger RNA level in nonreleasing human basophils. Am. J. Respir. Cell Mol. Biol. 23: 566-571
  6. Haisch, K. et al. (1999) Purification of morphologically and functionally intact human basophils to near homogeneity. J. Immunol. Methods 226: 129-137

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