Miltenyi Biotec-tested panel 19 (MBTP 19)
This application protocol describes the flow cytometric analysis of regulatory T cells (Tregs) after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD4||REA604||T helper cells||VioBlue®||450/50 (violet)|
|Viobility™ 405/520 Fixable Dye||-||Viability||-||525/50 (violet)|
|FoxP3||REA788||Regulatory T cells||Vio® B515||525/50 (blue)|
|CD357 (GITR)||REA980||Regulatory T cells||PE||585/40 (blue)|
|CD3e||REA606||T cells||PE-Vio® 615||655-730 (blue)|
|CD25||REA568||Regulatory T cells||PE-Vio 770||750LP (blue)|
|CD134 (OX40)||REA625||Regulatory T cells||APC||655-730 (red)|
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