Type of antibody:
Primary antibodies, Functional-grade antibodies
rat IgG1, rat IgG1κ

Specifications for IL-5 Antibody, anti-mouse


Interleukin 5 (IL-5) is pre-dominantly secreted by CD4
T cells. It is involved in a range of allergic reactions and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells.
The Anti-IL-5 antibody has been designed for intracellular staining of IL-5–producing cells. Cells can be stimulated for IL-5-production, for example, by polyclonal stimulation with mitogens. For induction of IL-5 production by antigen-specific T cells, cells are restimulated with respective antigen. IL-5 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL-5–producing cells can be stained intracellularly with Anti-IL-5 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL-5–producing cells. Magnetically enriched cells can be stained intracellularly for IL-5 production directly on the MACS
Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4
T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids.

Detailed product information

Technical specifications

Isotyperat IgG1κ, rat IgG1
Isotype controlIsotype Control Antibody, rat IgG1
Type of antibodyPrimary antibodies, Functional-grade antibodies
Molecular mass of antigen [kDa]13
Entrez Gene ID16191
RRIDAB_2660015, AB_2660016, AB_10829953

Resources for IL-5 Antibody, anti-mouse


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IL-5 Antibody, anti-mouse

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