Isolation and characterization of microglia

Application protocols

Discover our different workflows for microglia and find the one that fits your experimental needs:
 

Tissue dissociation of murine brain

Tissue dissociation into single-cell suspensions with high cell viability and a minimum of cell debris is a prerequisite for reliable cell separation, cell culture, and flow cytometry analysis. A dissociation procedure that combines optimized enzymatic treatment using our Tissue Dissociation Kits with gentle mechanical dissociation (gentleMACS) is crucial for the preparation of viable single-cell suspensions. 

Magnetic isolation and flow cytometric analysis of microglia

Our MACS® MicroBead Technology, in combination with the Adult Brain Dissociation Kit provides a gentle technique with minimal manipulation time. It allows you to obtain pure, viable and functional adult microglia.
 

Article
5 benefits of using magnetic cell separation to isolate neural cells from the central nervous system 
Read this recently published paper in PLoS ONE demonstrating the benefits of magnetic cell separation which include:

• Reliable isolation of different cell populations from CNS tissue across developmental time points, from postnatal and juvenile to adult
• Amendability to customization, using commercially available membrane-targeted antibodies 
• Retention of a dense network of cellular processes
• Enabled analysis of extrasomatic proteins