Preparation of single-cell and single-nuclei suspensions for genomic analysis
The generation of single-cell or single-nuclei suspensions from solid tissues is the most critical step prior to single-cell or single-nuclei sequencing. gentleMACS Technology represents a gentle, yet efficient approach to either extract viable cells or nuclei from different solid tissues. Whether tissues are fresh or frozen, extraction of nuclei is fast and easy with the Nuclei Extraction Buffer. Protocols for tissue dissociation and nuclei extraction have been optimized for speed and efficiency to give reliable results in downstream genomic analyses.
Single-cell analysis of dissociated whole mouse brain
Clear distinction of different neural cell populations from neonatal mouse brain in single-cell gene expression analysis, including all different major neural populations.
Analysis of tumor heterogeneity
Single-cell gene expression analysis of mouse tumors enables the clear identification of the different cellular populations present in the tissue.
Preparation of single-cell suspensions from solid tissue
Single-cell suspensions from fresh tissues can be easily generated using gentleMACS Technology, which combines gentle mechanical disruption with mild enzymatic digestion. gentleMACS Technology allows retrieval of different cell types present in the tissue, using automated and standardized protocols for reproducible results.
Video protocols for tissue dissociation
Find out how to standardize tissue dissociation using gentleMACS™ Technology step-by-step.
Preparation of single-nuclei suspensions from solid tissue
Single-nuclei suspensions can be easily generated from different types of solid tissues using the Nuclei Extraction Buffer in combination with the gentleMACS Octo Dissociators. This combination allows a gentle mechanical disruption of the tissues and an efficient cell lysis to release intact nuclei from cells in only seven minutes and of up to eight samples in parallel at low temperature.
High nuclei yields from mouse brain and tumors
Single-nuclei suspensions obtained from fresh and snap frozen mouse brain and syngeneic tumors show high numbers of extracted nuclei per mg of dissociated tissue.
High nuclei yields from different snap frozen mouse tissues and OCT embedded human tumors
Single-nuclei suspensions obtained from different snap frozen mouse tissues and OCT embedded human tumors show high numbers of extracted nuclei per mg of dissociated tissue.
Microscopy analysis of nuclei extracted from mouse brain
Intact nuclei from snap-frozen mouse brain extracted using the Nuclei Extraction Buffer in combination with the gentleMACS Octo Dissociator with Heaters.
Video
gentleMACS Dissociators: Reliability built in
Preparing single-cell and single-nuclei suspensions is fast and efficient with gentleMACS Dissociators. Genomic analysis results are more dependable because of the standardization and automation of gentleMACS Technology protocols.
