Cookie Settings
We use cookies to provide the best possible website experience for you. This includes cookies that are technically required to ensure a proper functioning of the website, as well as cookies which are used solely for anonymous statistical purposes, for more comfortable website settings, or for displaying personalized content. You are free to choose the categories you would like to permit. Please note that depending on your settings, the full functionality of the website may no longer be available. Further information can be found in our Privacy Statement and Cookie Statement.
PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. |
Stimulated sample |
Before enrichment | After enrichment |
IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 1PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. | IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 1PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. |
Unstimulated control |
Before enrichment | After enrichment |
IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 1PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. | IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 1PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. |
* Percentage represents frequency among CD4+ T cells. |
** Percentage represents frequency among enriched cells. |
PBMCs of a CMV + donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4 + T cells. |
Stimulated sample | Unstimulated control |
IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 2PBMCs of a CMV + donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4 + T cells. | IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 2PBMCs of a CMV + donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4 + T cells. |
* Percentage represents frequency among CD4+ T cells. |
PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. |
Stimulated sample |
Before enrichment | After enrichment |
IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 1PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. | IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 1PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. |
Unstimulated control |
Before enrichment | After enrichment |
IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 1PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. | IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 1PBMCs of a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. |
* Percentage represents frequency among CD4+ T cells. |
** Percentage represents frequency among enriched cells. |
PBMCs of a CMV + donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4 + T cells. |
Stimulated sample | Unstimulated control |
IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 2PBMCs of a CMV + donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4 + T cells. | IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanFigure 2PBMCs of a CMV + donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4 + T cells. |
* Percentage represents frequency among CD4+ T cells. |
Seems like you are coming from USA!
Do you want to visit our website in your country?
Copyright © 2022 Miltenyi Biotec and/or its affiliates. All rights reserved.