IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

Name: IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human
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IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

The IL-10 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-10-secreting cells.

Product data and images for IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

PBMCs of a CMV

⁺

donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit.

In the stimulated sample, 329 IL-10-secreting CD4

⁺

cells were enriched per 10

⁶

CD4

⁺

T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4

⁺

T cells were detected per 10

⁶

CD4

⁺

T cells after enrichment.

Stimulated sample

Before enrichment	After enrichment
View details IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human Figure 1 PBMCs of a CMV ⁺ donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 ⁺ cells were enriched per 10 ⁶ CD4 ⁺ T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 ⁺ T cells were detected per 10 ⁶ CD4 ⁺ T cells after enrichment.	View details IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human Figure 1 PBMCs of a CMV ⁺ donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 ⁺ cells were enriched per 10 ⁶ CD4 ⁺ T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 ⁺ T cells were detected per 10 ⁶ CD4 ⁺ T cells after enrichment.
Unstimulated control

Before enrichment	After enrichment
View details IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human Figure 1 PBMCs of a CMV ⁺ donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 ⁺ cells were enriched per 10 ⁶ CD4 ⁺ T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 ⁺ T cells were detected per 10 ⁶ CD4 ⁺ T cells after enrichment.	View details IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human Figure 1 PBMCs of a CMV ⁺ donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 329 IL-10-secreting CD4 ⁺ cells were enriched per 10 ⁶ CD4 ⁺ T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4 ⁺ T cells were detected per 10 ⁶ CD4 ⁺ T cells after enrichment.
* Percentage represents frequency among CD4⁺ T cells.

Figure 2

PBMCs of a CMV

⁺

donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4

⁺

T cells.

Stimulated sample	Unstimulated control
View details IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human Figure 2 PBMCs of a CMV ⁺ donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4 ⁺ T cells.	View details IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human Figure 2 PBMCs of a CMV ⁺ donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4 ⁺ T cells.
* Percentage represents frequency among CD4⁺ T cells.

Specifications for IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

Overview

The IL-10 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-10-secreting cells.

Detailed product information

Background information

Interleukin-10 (IL-10) is predominantly secreted by human CD4

⁺

T cells and antigen-presenting cells, such as monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by T

_H

1 cells, activated monocytes, and NK cells. It can stimulate the immunoglobulin production by B cells.

Downstream applications

IL-10 Secretion Assays were used to analyze IL-10-secreting, CMV-specific CD4

⁺

T cells after stimulation with CMV lysate. The IL-10 Secretion Assay was also used to analyze IL-10 secretion by allergen-specific T cells.

^1,2

Furthermore, it was used for the detection and isolation of antigen-specific IL-10-secreting type I regulatory T cells from patients with an autoimmune skin disease. The isolated cells were cloned, expanded and further analyzed for their regulatory function.

³

Columns

MS, LS, or autoMACS

^®

Columns.

Resources for IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

Documents and Protocols

Application protocol

Expansion of antigen-specific cytokine-secreting T cells

Protocols

Detection and enrichment of cytokine-secreting cells from whole blood

Protocols

Detection and enrichment of cytokine-secreting cells

References for IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

Publications

Akdis, M. et al. (2004) Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. J. Exp. Med. 199: 1567-1575
Reefer, A. J. et al. (2004) A role for IL-10-mediated HLA-DR7-restricted T cell-dependent events in development of the modified Tʜ2 response to cat allergen. J. Immunol. 172: 2763-2772
Veldman, C. et al. (2004) Type I regulatory T cells specific for desmoglein 3 are more frequently detected in healthy individuals than in patients with pemphigus vulgaris. J. Immunol. 172: 6468-6475

Data and images