Clone:
REAL262
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
Siglec-1, SN, Sialic acid-binding Ig-like lectin 1 (Siglec-1)

Specifications for CD169 (Siglec-1) Antibody, anti-mouse, REAdye_lease™

Overview

Clone REAL262 is an antibody fragment derived from the full CD169 (Siglec-1) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL262 recognizes the mouse CD169 (Siglec-1) antigen, a Single-pass type I membrane protein also known as Sialoadhesin or Sialic acid-binding Ig-like lectin 1 (Siglec-1). CD169 (Siglec-1) is a macrophage-restricted cell surface receptor that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8
+
T-cells. It is a member of the sialic acid-binding IgG-like lectin (Siglec) family of proteins characterized by affinity to specifically sialylated ligands, and under normal conditions is expressed on subsets of macrophages in secondary lymphoid tissues, such as lymph node and spleen. However, CD169
+
macrophages can also be found in a variety of pathological conditions, including (autoimmune) inflammatory infiltrates and tumors. CD169 (Siglec-1) has been shown to contribute to sialylated pathogen uptake, antigen presentation and lymphocyte proliferation, and to influence both immunity and tolerance. Additionally, CD169 (Siglec-1) positive macrophages, along with mesenchymal stem cells and β-adrenergic neurons, form the hematopoietic stem cell niche in the bone marrow. CD169
+
macrophages mediate signaling between the various cells and seem to promote hematopoietic stem cell retention to the niche.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

Siglec-1, SN, Sialic acid-binding Ig-like lectin 1 (Siglec-1)

Detailed product information

Technical specifications

CloneREAL262
Clonalitymonoclonal
Isotype controlControl Antibody
Hosthuman cell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD169 (Siglec-1)
Alternative names of antigenSiglec-1, SN, Sialic acid-binding Ig-like lectin 1 (Siglec-1)
Distribution of antigenmacrophages

Resources for CD169 (Siglec-1) Antibody, anti-mouse, REAdye_lease™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD169 (Siglec-1) Antibody, anti-mouse, REAdye_lease™

Publications

  1. Crocker, P. R. et al. (1994) Sialoadhesin, a macrophage sialic acid binding receptor for haemopoietic cells with 17 immunoglobulin-like domains. EMBO J. 13(19): 4490-4503
  2. Chow, A. et al. (2011) Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J. Exp. Med. 208(2): 261-271
  3. Saunderson, S. C. et al. (2014) CD169 mediates the capture of exosomes in spleen and lymph node. Blood 123(2): 208-216

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