Clone:
REA378
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, ICC, IHC, MC
Alternative names:
ITGAL, LFA-1, LFA1A, Leukocyte function-associated molecule 1 α chain

Extended validation for CD11a Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA378
HI111++
TS2/4-
MEM-25-
G43-25B-
Cells were incubated with an excess of purified unconjugated CD11a (REA378) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11a (REA378). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11a (REA378). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD11a (REA378). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD11a Antibody, anti-human, REAfinity™

Overview

Clone REA378 recognizes the human CD11a antigen, a single-pass type I membrane protein also known as integrin α-L (ITGAL). Integrins are heterodimeric adhesion molecules comprised of α- and β-subunits that participate in immune cell interactions as well as in immune cell-extracellular matrix interactions. CD11a combines with CD18 (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is a receptor for CD54 (ICAM-1), CD102 (ICAM-2), CD50 (ICAM-3), and CD242 (ICAM-4). It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T cell–mediated killing, and antibody dependent killing by granulocytes and monocytes. Interactions between LFA-1 and CD54 (ICAM-1) play a critical role in T cell–B cell collaboration.
Additional information: Clone REA378 displays negligible binding to Fc receptors.

Alternative names

ITGAL, LFA-1, LFA1A, Leukocyte function-associated molecule 1 α chain

Detailed product information

Technical specifications

CloneREA378
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate, other
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
chimpanzee (
Pan troglodytes
)
,
olive baboon (
Papio anubis
)
, capuchin monkey, horse, cattle (bovine, cow), sheep, dog, rabbit
AntigenCD11a
Alternative names of antigenITGAL, LFA-1, LFA1A, Leukocyte function-associated molecule 1 α chain
Molecular mass of antigen [kDa]126
Distribution of antigenleukocytes, T cells, granulocytes, monocytes
Entrez Gene ID3683
RRIDAB_2904688, AB_2904687, AB_2654622, AB_2654623, AB_2654630, AB_2654631, AB_2889429, AB_2889428, AB_2904686, AB_2904685, AB_2904684, AB_2904690, AB_2904689, AB_2783916, AB_2889431, AB_2889430, AB_2819706, AB_2819698, AB_2801921, AB_2783917

Resources for CD11a Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD11a Antibody, anti-human, REAfinity™

Publications

  1. Lowes, M. A. et al. (2005) Increase in TNF-alpha and inducible nitric oxide synthase-expressing dendritic cells in psoriasis and reduction with efalizumab (anti-CD11a). Proc. Natl. Acad. Sci. U.S.A. 102(52): 19057-19062
  2. Larson, R. S. et al. (1989) Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily. J. Cell Biol. 108(2): 703-712
  3. Zhou, Y. et al. (2012) Usefulness of CD11a and CD18 in flow cytometric immunophenotypic analysis for diagnosis of acute promyelocytic leukemia. Am. J. Clin. Pathol. 138(5): 744-750

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