Dissociation, culture, and analysis of cerebral organoids

This application protocol describes the step-by-step dissociation of PSC-derived cerebral organoids into single cells, using the Neural Tissue Dissociation Kit (P) or (T) in combination with the gentleMACS™ Dissociator. After dissociation, single cells can be either analyzed by flow cytometry using the MACSQuant® Analyzer 10 or can be cultivated for immunostaining and microscopy analysis.

Protocol

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This protocol includes detailed instructions for preparing necessary reagents and materials and then carrying out each step.

Materials

The following is a listing of reagents, instruments, and consumables needed for each step of this protocol. These products are for research use only.
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