Miltenyi Biotec-tested panel 31 (MBTP 31)
This application protocol describes the flow cytometric analysis of the expression of transcription factors by T cell subsets from human PBMCs.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD45RA||REA562||Leukocytes||PE-Vio® 615||655-730 (blue)|
|CD4||REA623||T helper cells||APC-Vio 770||750 LP (red)|
|CD8||REA734||Cytotoxic T cells||VioGreen™||525/50 (violet)|
|CD25||REA570||Regulatory T cells||PE||585/40 (blue)|
|CD127||REA614||Cell type exclusion||PE-Vio 770||750 LP (blue)|
|Helios||REA829||Transcription factor||VioBlue®||450/50 (violet)|
|Ki-67||REA183||Transcription factor||Vio B515||525/50 (blue)|
|T-bet||REA102||Transcription factor||APC||655-730 (red)|
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