Tissue dissociation into single-cell suspensions with high cell viability and a minimum of cell debris is a prerequisite for reliable cell separation, cell culture, and flow cytometry analysis. A dissociation procedure that combines optimized enzymatic treatment using our Tissue Dissociation Kits with gentle mechanical dissociation provided by our gentleMACS™ Dissociators is crucial for the preparation of viable single-cell suspensions.
|Tissue dissociation kits||Starting material|
|Isolation of all neural cells|
|Adult Brain Dissociation Kit, mouse and rat||Animal age > P7|
|Neural Tissue Dissociation Kit (P)||Animal age ≤ P7|
|Neural Tissue Dissociation Kit (T)||Animal age ≤ P7|
|Isolation of neurons only|
|Neural Tissue Dissociation Kit – Postnatal Neurons||Animal age ≤ P7|
|Brain Tumor Dissociation Kit (P)||Brain tumor tissue|
|Neurosphere Dissociation Kit (P)||Cultured neurospheres|
|Embryoid Body Dissociation Kit, human and mouse||In vitro generated embryoid bodies|
|Myelin Removal Beads II, human, mouse, rat||Single-cell suspensions|
|(P) Papain, (T) Trypsin|
When working with neural tissue derived from > P7 animals, large amounts of cell debris and erythrocytes often hurdle downstream applications. The Adult Brain Dissociation Kit, mouse and rat comes with debris and red blood cell (RBC) removal solutions that overcome this problem.