Checking the quality of your samples before your genomic analysis helps not only to set the right expectations on the outcome of the analysis, but also to save time and costs to avoid using poor quality samples in the analysis. In particular, for single-cell or single-nuclei analysis, it is especially important to perform an accurate cell and nuclei count, check that samples are in a uniform suspension, have high cell viability or intact nuclei, and contain as little debris as possible. The MACSQuant® Analyzer flow cytometers allow you to check all these parameters at once with absolute and fast volumetric cell counting and analysis.
Accurate flow cytometric analysis of dissociated tumors checks the quality of the cell suspension prior to single-cell genomic analysis and sets up the right standards.After dissociation of the tumor using the Tumor Dissociation Kit, human and gentleMACS™ Octo Dissociator with Heaters, TILs were analyzed by flow cytometry using the 8-Color Immunophenotyping Kit and MACSQuant Analyzer 16.
The data shows exemplary quality control analysis of a suspension of neural cells after dissociation of mouse brain and analyzed using MACSQuant Analyzer 10.
The MACSQuant Analyzers make flow cytometry easy for any user. With their unique function of volumetric pipetting, absolute cell counting is automatically provided for every sample acquired and every population analyzed. It only takes a few seconds to obtain accurate cell counts on each sample, and using as little as 25 µL of your sample.
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